Green synthesis of silver nanoparticles through oil: Promoting full-thickness cutaneous wound healing in methicillin-resistant Staphylococcus aureus infections

• Published in: Frontiers in bioengineering and biotechnology 2022-08, Vol.10, p.856651
• Main Authors: Wang, Yuhan, Li, Qinmei, Peng, Xiaomin, Li, Zheng, Xiang, Jun, Chen, Yunru, Hao, Kaiyuan, Wang, Shuaiyang, Nie, Dongyang, Cui, Yao, Lv, Feifei, Wang, Ying, Wu, Wenda, Guo, Dawei, Si, Hongbin
• Format: Article
• Language: English
• Subjects: AgNPs; antibacterial; Bioengineering and Biotechnology; green synthesis; L. cubeba oil; multidrugresistance; wound infection
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2022.856651

Due to the emergence of multi-drug resistant microorganisms, the development and discovery of alternative eco-friendly antimicrobial agents have become a top priority. In this study, a simple, novel, and valid green method was developed to synthesize essential oil-silver nanoparticles (Lceo-AgNPs) using Lceo as a reducing and capping agent. The maximum UV absorbance of Lceo-AgNPs appeared at 423 nm and the size was 5-15 nm through transmission electron microscopy result. The results of Fourier transform infrared and DLS showed that Lceo provided sufficient chemical bonds for Lceo-AgNPs to reinforce its stability and dispersion. The antibacterial effects of Lceo-AgNPs against microbial susceptible multidrug-resistant and were determined. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Lceo-AgNPs against were 25 and 50 μg/ml. The MIC and MBC of Lceo-AgNPs against MRSA were 50 and 100 μg/ml, respectively. The results of scanning electron microscopy showed that the amount of bacteria obviously decreased and the bacteria cells were destroyed by Lceo-AgNPs. research disclosed significant wound healing and re-epithelialization effects in the Lceo-AgNPs group compared with the self-healing group and the healing activity was better than in the sulfadiazine silver group. In this experiment, Lceo-AgNPs has been shown to have effects on killing multidrug-resistant bacteria and promoting wound healing. This study suggested Lceo-AgNPs as an excellent new-type drug for wound treatment infected with multidrug-resistant bacteria, and now expects to proceed with clinical research.