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Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate
Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood-brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microgl...
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| Published in: | Cells (Basel, Switzerland) Switzerland), 2022-08, Vol.11 (15), p.2393 |
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| Main Authors: | , , , , , , , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c481t-bb192185c4717b51de37315175b3fea4992f311ca30132371ab2aaba81bb96503 |
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| cites | cdi_FETCH-LOGICAL-c481t-bb192185c4717b51de37315175b3fea4992f311ca30132371ab2aaba81bb96503 |
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| container_issue | 15 |
| container_start_page | 2393 |
| container_title | Cells (Basel, Switzerland) |
| container_volume | 11 |
| creator | Shin, Tae Hwan Lee, Da Yeon Jang, Yong Eun Kwon, Do Hyeon Hwang, Ji Su Kim, Seok Gi Seo, Chan Paik, Man Jeong Lee, Ju Yeon Kim, Jin Young Park, Seokho Choi, Sung-E Basith, Shaherin Kim, Myeong Ok Lee, Gwang |
| description | Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood-brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microglia, the resident immune cells in the brain, which are exposed to nanoparticles. We analysed the cytotoxic effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO
(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis. |
| doi_str_mv | 10.3390/cells11152393 |
| format | article |
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(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.</description><identifier>ISSN: 2073-4409</identifier><identifier>EISSN: 2073-4409</identifier><identifier>DOI: 10.3390/cells11152393</identifier><identifier>PMID: 35954236</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Artificial intelligence ; Blood-brain barrier ; Brain research ; Citrates ; Citric Acid ; Cytotoxicity ; Exocytosis ; Gene expression ; Immune response ; integrated omics ; Isothiocyanate ; Leukocyte migration ; Magnetite Nanoparticles ; Metabolomics ; Microglia ; Nanoparticles ; nanotoxicology ; Neurodegeneration ; Ontology ; Proteomics ; Quantum dots ; Rhodamine ; Silicon dioxide ; Silicon Dioxide - pharmacology ; Software ; Toxicology ; Transcriptomics</subject><ispartof>Cells (Basel, Switzerland), 2022-08, Vol.11 (15), p.2393</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-bb192185c4717b51de37315175b3fea4992f311ca30132371ab2aaba81bb96503</citedby><cites>FETCH-LOGICAL-c481t-bb192185c4717b51de37315175b3fea4992f311ca30132371ab2aaba81bb96503</cites><orcidid>0000-0002-1299-9478 ; 0000-0001-9686-4556 ; 0000-0003-0855-6516</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2700533636/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2700533636?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35954236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Tae Hwan</creatorcontrib><creatorcontrib>Lee, Da Yeon</creatorcontrib><creatorcontrib>Jang, Yong Eun</creatorcontrib><creatorcontrib>Kwon, Do Hyeon</creatorcontrib><creatorcontrib>Hwang, Ji Su</creatorcontrib><creatorcontrib>Kim, Seok Gi</creatorcontrib><creatorcontrib>Seo, Chan</creatorcontrib><creatorcontrib>Paik, Man Jeong</creatorcontrib><creatorcontrib>Lee, Ju Yeon</creatorcontrib><creatorcontrib>Kim, Jin Young</creatorcontrib><creatorcontrib>Park, Seokho</creatorcontrib><creatorcontrib>Choi, Sung-E</creatorcontrib><creatorcontrib>Basith, Shaherin</creatorcontrib><creatorcontrib>Kim, Myeong Ok</creatorcontrib><creatorcontrib>Lee, Gwang</creatorcontrib><title>Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate</title><title>Cells (Basel, Switzerland)</title><addtitle>Cells</addtitle><description>Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood-brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microglia, the resident immune cells in the brain, which are exposed to nanoparticles. We analysed the cytotoxic effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO
(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.</description><subject>Artificial intelligence</subject><subject>Blood-brain barrier</subject><subject>Brain research</subject><subject>Citrates</subject><subject>Citric Acid</subject><subject>Cytotoxicity</subject><subject>Exocytosis</subject><subject>Gene expression</subject><subject>Immune response</subject><subject>integrated omics</subject><subject>Isothiocyanate</subject><subject>Leukocyte migration</subject><subject>Magnetite Nanoparticles</subject><subject>Metabolomics</subject><subject>Microglia</subject><subject>Nanoparticles</subject><subject>nanotoxicology</subject><subject>Neurodegeneration</subject><subject>Ontology</subject><subject>Proteomics</subject><subject>Quantum dots</subject><subject>Rhodamine</subject><subject>Silicon dioxide</subject><subject>Silicon Dioxide - pharmacology</subject><subject>Software</subject><subject>Toxicology</subject><subject>Transcriptomics</subject><issn>2073-4409</issn><issn>2073-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdksFv0zAUxiMEYlPZkSuyxIVLwM8vTuIL0lQNNmkDaWxny3ac1FUaFzst6pl_fE5bphX74KfPn35-z-9l2XugnxEF_WJs30cA4AwFvsrOGa0wLwoqXr-Iz7KLGJc0rRpKoPxtdoZc8IJheZ79vbfNxozOD8QNZFxYcue6oPbCZdK3btwR3ybVBN_1TpGHYNVoG_LHjQvyy_XOqHzu99Kd6gY7OkN-qMGvVUhhbyNRQzORXSD31vitDTvyGN3Qkbkb01P2XfamVX20F8dzlj1-u3qYX-e3P7_fzC9vc1PUMOZag2BQc1NUUGkOjcUKgUPFNbZWFUKwFgGMQgrIsAKlmVJa1aC1KDnFWXZz4DZeLeU6uJUKO-mVk3vBh04ec5YtWK0nBNd1YRTUBdOCN9paVnBs2sT6emCtN3plG2OHVEp_Aj29GdxCdn4rBZZ1UdYJ8OkICP73xsZRrlyc-qkG6zdRsooyEMjZZP34n3XpN2FIXzW5KEcs055l-cGVGhVjsO1zMkDlNC3yZFqS_8PLCp7d_2YDnwAFWbya</recordid><startdate>20220803</startdate><enddate>20220803</enddate><creator>Shin, Tae Hwan</creator><creator>Lee, Da Yeon</creator><creator>Jang, Yong Eun</creator><creator>Kwon, Do Hyeon</creator><creator>Hwang, Ji Su</creator><creator>Kim, Seok Gi</creator><creator>Seo, Chan</creator><creator>Paik, Man Jeong</creator><creator>Lee, Ju Yeon</creator><creator>Kim, Jin Young</creator><creator>Park, Seokho</creator><creator>Choi, Sung-E</creator><creator>Basith, Shaherin</creator><creator>Kim, Myeong Ok</creator><creator>Lee, Gwang</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1299-9478</orcidid><orcidid>https://orcid.org/0000-0001-9686-4556</orcidid><orcidid>https://orcid.org/0000-0003-0855-6516</orcidid></search><sort><creationdate>20220803</creationdate><title>Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate</title><author>Shin, Tae Hwan ; 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(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35954236</pmid><doi>10.3390/cells11152393</doi><orcidid>https://orcid.org/0000-0002-1299-9478</orcidid><orcidid>https://orcid.org/0000-0001-9686-4556</orcidid><orcidid>https://orcid.org/0000-0003-0855-6516</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Cells (Basel, Switzerland), 2022-08, Vol.11 (15), p.2393 |
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| language | eng |
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| subjects | Artificial intelligence Blood-brain barrier Brain research Citrates Citric Acid Cytotoxicity Exocytosis Gene expression Immune response integrated omics Isothiocyanate Leukocyte migration Magnetite Nanoparticles Metabolomics Microglia Nanoparticles nanotoxicology Neurodegeneration Ontology Proteomics Quantum dots Rhodamine Silicon dioxide Silicon Dioxide - pharmacology Software Toxicology Transcriptomics |
| title | Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate |
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