Extraction, Characterization and Osteogenic Activity of a Type I Collagen from Starfish ( Asterias amurensis )

• Published in: Marine drugs 2023-04, Vol.21 (5), p.274
• Main Authors: Li, Lingcui, Yu, Yu, Wu, Wenhui, Wang, Peipei
• Format: Article
• Language: English
• Subjects: Alanine; Alkaline phosphatase; Amino acid composition; Amino acids; Animals; Aquaculture; Asterias - chemistry; Asterias amurensis; Bone marrow; Bones; Calcification; Cell Differentiation; Cell proliferation; Chemical properties; Chromatography; Collagen; Collagen Type I; Differentiation (biology); Ecosystem; Epidemics; Extraction (Chemistry); Functional foods; Functional foods & nutraceuticals; Gene expression; Glycine; Glycine (amino acid); Growth; Health aspects; Hydrogen bonds; Hydroxyproline; Marine ecosystems; Marine resources; Melt temperature; Methods; Mice; Microstructural analysis; Microstructure; Mineralization; mRNA; Nodules; Osteogenesis; osteogenic differentiation; Peptides; Pharmacology, Experimental; Phosphatase; Polyculture (aquaculture); Proliferation; Protein composition; Protein structure; Proteins; Secondary structure; Shellfish; starfish; Starfish - chemistry; Stem cells; Thermal stability; type I collagen; Ultraviolet spectra; Vibration
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21050274

Outbreaks of starfish ( ) pose a major threat to aquaculture and marine ecosystems in Qingdao, China, and no effective methods have been found to control them. A comprehensive study of collagen in starfish could be an alternative to high efficient utilization. Based on this, collagen was firstly extracted from Qingdao . Then, its protein pattern, amino acid composition, secondary structure, microstructure and thermal stability were investigated. The results showed that the collagen (AAC) is a type I collagen composed of α , α , and β chains. Glycine, hydroxyproline, and alanine were the major amino acids. The melting temperature was 57.7 °C. From FTIR, UV spectra and CD chromatography, the AAC had an intact triple helix and secondary structure, and microstructural analysis showed that the AAC had a loose, fibrous porous structure. Next, the osteogenic differentiation effect of AAC on Mouse bone marrow stem cells (BMSCs) was investigated, and the results showed that AAC induced osteogenic differentiation of cells by promoting the proliferation of BMSCs, enhancing alkaline phosphatase (ALP) activity, promoting cell mineralization nodules and upregulating the expression of mRNA of relevant osteogenic genes. These results suggest that AAC might have the potential application to bone health-related functional foods.