Hypertonic Salt Solution Enhances Inflammatory Responses in Cultured Splenic T-Cells from Dahl Salt-Sensitive Rats but Not Dahl Salt-Resistant Rats

This study aimed to delineate the effect of sodium chloride on the induction of inflammatory responses and the development of hypertension in Dahl salt-sensitive (SS) and salt-resistant (SR) rats. Splenocytes were isolated from the spleens of SS and SR rats, and cultured on anti-CD3-coated plates fo...

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Bibliographic Details
Published in:Journal of cardiovascular development and disease 2023-10, Vol.10 (10), p.414
Main Authors: Jang, Sungmin, Kim, Jee Young, Kim, Cheong-Wun, Kim, Inkyeom
Format: Article
Language:English
Subjects:
Antibodies
Blood pressure
Cell culture
Cloning
Cytokines
Dahl salt-resistant rats
Dahl salt-sensitive rats
Enzyme-linked immunosorbent assay
Flow cytometry
Growth factors
Hypertension
hypertonic salt solution
Kinases
Laboratory animals
Nitric oxide
Salt
salt sensitivity
Sodium
Software
T cells
Th17 cells
Transcription factors
Transforming growth factors
Treg cells
Tumor necrosis factor-TNF
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Summary:This study aimed to delineate the effect of sodium chloride on the induction of inflammatory responses and the development of hypertension in Dahl salt-sensitive (SS) and salt-resistant (SR) rats. Splenocytes were isolated from the spleens of SS and SR rats, and cultured on anti-CD3-coated plates for 5 days. The cultured splenic T-cells were challenged with a hypertonic salt solution (0, 20, or 40 mM) in the absence or presence of IL-6 (0, 20, or 60 ng/mL), TGF-β (0, 5, or 15 ng/mL), or IL-23 (0, 10, or 30 ng/mL), and analyzed via ELISA, flow cytometry, and immunofluorescence. The hypertonic salt solution potentiated IL-17A production, as well as the differentiation of Th17 cells via IL-6/TGF-β/IL-23, exclusively in SS rats. However, it did not affect IL-10 production or the differentiation of Treg cells in any of the groups. Furthermore, it potentiated the signal of RORγt in IL-6-treated splenic T-cells from SS rats. To summarize, cultured splenic T-cells exhibited enhanced inflammatory responses on exposure to a hypertonic salt solution in SS rats only, which indicated that sodium chloride and inflammatory cytokines synergistically drove the induction of pathogenic Th17 cells and the development of hypertension in this group only.
ISSN:2308-3425
2308-3425
DOI:10.3390/jcdd10100414