Hypertonic Salt Solution Enhances Inflammatory Responses in Cultured Splenic T-Cells from Dahl Salt-Sensitive Rats but Not Dahl Salt-Resistant Rats

• Published in: Journal of cardiovascular development and disease 2023-10, Vol.10 (10), p.414
• Main Authors: Jang, Sungmin, Kim, Jee Young, Kim, Cheong-Wun, Kim, Inkyeom
• Format: Article
• Language: English
• Subjects: Antibodies; Blood pressure; Cell culture; Cloning; Cytokines; Dahl salt-resistant rats; Dahl salt-sensitive rats; Enzyme-linked immunosorbent assay; Flow cytometry; Growth factors; Hypertension; hypertonic salt solution; Kinases; Laboratory animals; Nitric oxide; Salt; salt sensitivity; Sodium; Software; T cells; Th17 cells; Transcription factors; Transforming growth factors; Treg cells; Tumor necrosis factor-TNF
• ISSN: 2308-3425; 2308-3425
• DOI: 10.3390/jcdd10100414

This study aimed to delineate the effect of sodium chloride on the induction of inflammatory responses and the development of hypertension in Dahl salt-sensitive (SS) and salt-resistant (SR) rats. Splenocytes were isolated from the spleens of SS and SR rats, and cultured on anti-CD3-coated plates for 5 days. The cultured splenic T-cells were challenged with a hypertonic salt solution (0, 20, or 40 mM) in the absence or presence of IL-6 (0, 20, or 60 ng/mL), TGF-β (0, 5, or 15 ng/mL), or IL-23 (0, 10, or 30 ng/mL), and analyzed via ELISA, flow cytometry, and immunofluorescence. The hypertonic salt solution potentiated IL-17A production, as well as the differentiation of Th17 cells via IL-6/TGF-β/IL-23, exclusively in SS rats. However, it did not affect IL-10 production or the differentiation of Treg cells in any of the groups. Furthermore, it potentiated the signal of RORγt in IL-6-treated splenic T-cells from SS rats. To summarize, cultured splenic T-cells exhibited enhanced inflammatory responses on exposure to a hypertonic salt solution in SS rats only, which indicated that sodium chloride and inflammatory cytokines synergistically drove the induction of pathogenic Th17 cells and the development of hypertension in this group only.