Polyphenol-Rich Fraction of Ecklonia cava Improves Nonalcoholic Fatty Liver Disease in High Fat Diet-Fed Mice

• Published in: Marine drugs 2015-11, Vol.13 (11), p.6866-6883
• Main Authors: Park, Eun-Young, Choi, Hojung, Yoon, Ji-Young, Lee, In-Young, Seo, Youngwan, Moon, Hong-Seop, Hwang, Jong-Hee, Jun, Hee-Sook
• Format: Article
• Language: English
• Subjects: Animals; Cholesterol 7-alpha-Hydroxylase - genetics; Cytokines - genetics; Diet, High-Fat; Disease Models, Animal; Ecklonia cava; fatty liver; Gene Expression Regulation - drug effects; hepatic steatosis; Inflammation - drug therapy; Inflammation - pathology; Lipid Metabolism - drug effects; Lipogenesis - drug effects; Lipogenesis - genetics; Liver - drug effects; Liver - pathology; Male; Marine; Mice; Mice, Inbred C57BL; MRI; MRS; NAFLD; Non-alcoholic Fatty Liver Disease - drug therapy; Obesity - metabolism; Phaeophyceae - chemistry; Polyphenols - isolation & purification; Polyphenols - pharmacology; RNA, Messenger - metabolism
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md13116866

Ecklonia cava (E. cava; CA) is an edible brown alga with beneficial effects in diabetes via regulation of various metabolic processes such as lipogenesis, lipolysis, inflammation, and the antioxidant defense system in liver and adipose tissue. We investigated the effect of the polyphenol-rich fraction of E. cava produced from Gijang (G-CA) on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed mice. C57BL6 mice were fed a HFD for six weeks and then the HFD group was administered 300 mg/kg of G-CA extracts by oral intubation for 10 weeks. Body weight, fat mass, and serum biochemical parameters were reduced by G-CA extract treatment. MRI/MRS analysis showed that liver fat and liver volume in HFD-induced obese mice were reduced by G-CA extract treatment. Further, we analyzed hepatic gene expression related to inflammation and lipid metabolism. The mRNA expression levels of inflammatory cytokines and hepatic lipogenesis-related genes were decreased in G-CA-treated HFD mice. The mRNA expression levels of cholesterol 7 alpha-hydroxylase 1 (CYP7A1), the key enzyme in bile acid synthesis, were dramatically increased by G-CA treatment in HFD mice. We suggest that G-CA treatment ameliorated hepatic steatosis by inhibiting inflammation and improving lipid metabolism.