New Insight into the Composition of Wheat Seed Microbiota

Endophytes are associated with host plants throughout their life history from seed germination to fruit development. One of the most important plant organs colonized by endophytic microbiota is the seed. The aim of this study was to determine the structure of the seed core microbiome inhabiting the...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-06, Vol.21 (13), p.4634
Main Authors: Kuźniar, Agnieszka, Włodarczyk, Kinga, Grządziel, Jarosław, Woźniak, Małgorzata, Furtak, Karolina, Gałązka, Anna, Dziadczyk, Ewa, Skórzyńska-Polit, Ewa, Wolińska, Agnieszka
Format: Article
Language:English
Subjects:
Abundance
Bacteria
Biodiversity
core microbiome
Cultivars
Embryos
Endophytes
Endophytes - genetics
Endophytes - metabolism
endosperm
Germination
Host plants
Life history
Microbiomes
Microbiota
Microbiota - genetics
Microorganisms
Organs
Paenibacillus
Plant breeding
Propionibacterium
RNA, Ribosomal, 16S - genetics
Seed germination
Seeds
Seeds - genetics
Seeds - microbiology
Triticum - genetics
Triticum - microbiology
Triticum aestivum
Triticum spelta
Wheat
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Summary:Endophytes are associated with host plants throughout their life history from seed germination to fruit development. One of the most important plant organs colonized by endophytic microbiota is the seed. The aim of this study was to determine the structure of the seed core microbiome inhabiting the endosperms and embryos of eight wheat cultivars with the use of a culture-independent technique. The seeds of L. cv. Hondia, Wilejka, STH, Opcja, Tybalt, Euforia and L. cv. Rokosz and Schwabencorn (producer: Plant Breeding Strzelce Sp. z o.o. Group IHAR) were studied. Rokosz and Hondia were cultured in vitro and in vivo to identify obligatory bacterial endophytes. A restrictive analysis of reads originating from the in vitro plants has demonstrated that the bacterial genera and inhabiting Rokosz and Hondia plants have a status of obligatory microorganisms. Greater biodiversity of seed-borne endophytes was found in the seed endosperms than in the embryos. The multiple comparison analysis of the OTU abundance indicated that the seed part significantly influenced the relative abundance. The seed-born microbiome is not statistically significantly dependent on the wheat cultivars; however, it cannot be claimed that every wheat seed is the same.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21134634