Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan...

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Bibliographic Details
Published in:Virology journal 2021-02, Vol.18 (1), p.42-42, Article 42
Main Authors: Zhao, Kuangjie, Yin, Yueyan, Hua, Mengying, Wang, Shaoxiang, Mo, Xiaohan, Yuan, Enping, Zheng, Hongying, Lin, Lin, Chen, Hairu, Lu, Yuwen, Chen, Jianping, Peng, Jiejun, Yan, Fei
Format: Article
Language:English
Subjects:
Amino acids
Capsicum frutescens
Cloning
Diseases and pests
Genetic aspects
Genetic recombination
Genomes
Identification and classification
Laboratories
Next-generation sequencing
Nucleotide sequence
Nucleotides
Pepper vein yellows virus
Peppers
Phylogenetics
Plant viruses
Polerovirus
Polymerase chain reaction
Proteins
Readthrough domain
Recombination
RNA viruses
Short Report
Tobacco
Virus diseases of plants
Virus research
Viruses
Yellowing
Yellows
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Description
Summary:Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5' half of its genome (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3'-part encoding P5, where it is most similar (91.7% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event affecting nucleotide positions 4126 to 5192 nt, with PeVYV-3 as the major parent but with the region 4126-5192 nt derived from TVDV as the minor parent. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.
ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-021-01511-5