Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells

• Published in: Asian Pacific journal of tropical medicine 2018-03, Vol.11 (3), p.235-239
• Main Authors: Cheng, Na, Liu, Xiao-Ran, Liu, Da-Wei, Liu, Fang, Xiang, Jin, Yang, Di, Zhao, Guo-Qiang
• Format: Article
• Language: English
• Subjects: differentiation-inducing therapy; regenerated tissue extracts of liver injury; sk-hep1 cell enrichment (sk-hep1-fj; sk-hep1 hepatoma cell
• ISSN: 1995-7645; 2352-4146; 2352-4146
• DOI: 10.4103/1995-7645.228439

Objective: To study the effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells. Methods: Regenerated tissue extracts after liver injury were used to induce SK-HEP1 cells after enrichment, their effects on the proliferation, differentiation, migration and invasion of SK-HEP1 cells were observed through in vitro cell culture, MTT, flow cytometry and transwell assays. Results: In response to the action of regenerated tissue extracts after liver injury, SK-HEP1 cells were blocked in G0/G1 phase, their growth rate was distinctly reduced. The number of SK-HEP1- fj colonies decreased. The migration ability of SK-HEP1 cells showed a decreased trend on day 7 and day 11 after induction. SK-HEPl's invasion ability clearly decreased on days 7 and 11 after induction, especially on day 7. Conclusions: To a certain extent, regenerated tissue extracts after liver injury can inhibit the proliferation, differentiation, migration and invasion of hepatoma cells, showing an important potential of being a differentiating agent for the treatment of liver cancer.