SERS-based quantitative detection of ovarian cancer prognostic factor haptoglobin

Surface-enhanced Raman spectroscopy (SERS) is increasingly being used for biosensing because of its high sensitivity and low detection limit, which are made possible by the unique Raman 'fingerprint' spectra from the biomolecules. Here we propose a novel SERS method for the fast, sensitive...

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Bibliographic Details
Published in:International journal of nanomedicine 2015-01, Vol.10 (default), p.1831-1840
Main Authors: Perumal, Jayakumar, Balasundaram, Ghayathri, Mahyuddin, Aniza P, Choolani, Mahesh, Olivo, Malini
Format: Article
Language:English
Subjects:
Benzidines - chemistry
Biomarkers
Biomarkers, Tumor - analysis
Cysts
Diagnosis
Enzymes
Female
Genetic aspects
Haptoglobin
Haptoglobins - analysis
Haptoglobins - metabolism
Hemoglobins - metabolism
Humans
Hydrogen Peroxide - chemistry
Hydrogen Peroxide - metabolism
Measurement
Methods
Nanoparticles
Original Research
Ovarian cancer
Ovarian Neoplasms - metabolism
Peroxidases - chemistry
Peroxidases - metabolism
Physiological aspects
Prognosis
Properties
Proteins
Sensitivity and Specificity
Spectrum analysis
Spectrum Analysis, Raman - methods
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Summary:Surface-enhanced Raman spectroscopy (SERS) is increasingly being used for biosensing because of its high sensitivity and low detection limit, which are made possible by the unique Raman 'fingerprint' spectra from the biomolecules. Here we propose a novel SERS method for the fast, sensitive, and reliable quantitative analysis of haptoglobin (Hp), an acute phase plasma glycoprotein that is widely gaining application as a prognostic ovarian cancer biomarker. We exploited the peroxidase activity of the hemoglobin-haptoglobin (Hb-Hp) complex formed by the selective and specific binding of Hp to free Hb to catalyze the reaction of 3,3',5,5'-tetramethylbenzidine (TMB) substrate and hydrogen peroxide to result in the final product of strongly SERS-active TMB(2+). We observed a linear increase in the SERS signal of TMB(2+) with increasing concentrations of Hb-Hp complex from 50 nM to 34 μM. Based on this concentration-dependent SERS spectrum, we quantified Hp in clinical samples. We observed that our inference about the prognosis of the disease coincided with the histology data and that our method was much more sensitive than the enzyme-linked immunosorbent assay method.
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S75552