Apophysomyces jiangsuensis sp. nov., a Salt Tolerant and Phosphate-Solubilizing Fungus from the Tidelands of Jiangsu Province of China

A newly isolated phosphate-solubilizing fungus from the topsoil of habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The spores are ellipsoida...

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Bibliographic Details
Published in:Microorganisms (Basel) 2020-11, Vol.8 (12), p.1868
Main Authors: Li, Siyu, Han, Ruiming, Zhang, Huanshi, Song, Yongchun, Zhao, Fugeng, Qin, Pei
Format: Article
Language:English
Subjects:
Apophysomyces
Aquatic plants
Calcium phosphates
Citric acid
Coastal ecology
Deoxyribonucleic acid
DNA
Fermented food
Fungi
Genetic analysis
Genetic distance
Glucose
Lecithin
Low molecular weights
Microorganisms
Microscopy
Molecular weight
Morphology
New species
Organic acids
Oxalic acid
Phosphates
Phosphorus
phosphorus-dissolving
Phylogenetics
Salinity
Salt marshes
Scanning electron microscopy
Sequence analysis
Sodium chloride
soil fungi
Sporangia
Spores
taxonomy
Topsoil
Tricalcium phosphate
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Summary:A newly isolated phosphate-solubilizing fungus from the topsoil of habitats in Yancheng coastal salt marsh was cultivated. Scanning electron microscopy observation revealed that the sporangia are nearly spherical, peach-shaped, and the spores formed on the top of sporangia. The spores are ellipsoidal with raised white nubbins on the surface. Based on a polyphasic study and the genetic distance analysis referring to the sequence analysis of ITS (ITS1 + 5.8S + ITS2) and 28S rDNA (D1/D2 domains) genes, the novel species belongs to the genus and is named as . The optimum growth temperature and salinity of the new species were 28 °C and 1.15% NaCl, respectively. A study of its phosphate-solubilizing ability revealed that the fungus had an obvious decomposition effect on lecithin, Ca (PO ) , and AlPO , respectively. The pH of the fermented liquid progressively decreased from 6.85 to 2.27 after 7 days of incubation, indicating that the low molecular weight organic acids excreted into the culture liquor were oxalic, succinic, and malic acids and a trace amount of citric acid. Among these, oxalic acid was the major organic acid, and its amount reached 652.5 mg/L. These results indicated that the main mechanism underlying the dissolved phosphorus was related to the secretion of large amounts of organic acids.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8121868