α-tocopherol levels in natural and artificial aging of soybean seeds/Teores de α-tocoferol no envelhecimento natural e artificial de sementes de soja

• Published in: Acta scientiarum. Agronomy 2012-07, Vol.34 (3), p.339-346
• Main Authors: Giurizatto, Maria Izabel Kruger, Ferrarese-Filho, Osvaldo, Ferrarese, Maria de Lourdes Lucio, Robaina, Antonio Dias, Goncalves, Manoel Carlos, Cardoso, Claudia Andrea Lima
• Format: Article
• Language: eng ; spa
• Subjects: armazenamento; Glycine max (L.) Merrill; storage; α-tocoferol; α-tocopherol
• ISSN: 1679-9275; 1807-8621
• DOI: 10.4025/actasciagron.v34i3.12660

Tocopherols are well known constituents of vitamin E, and the main antioxidants in soybean. Are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. The objective of this work was to determine the level of α-tocopherol in four soybean seeds cultivars naturally and artificially aged. Seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for α-tocopherol content. The quantification of α-tocopherol in the soybean seeds was performed by high performance liquid chromatography (HPLC) combined with the Soxhlet extraction method. Significant differences in α-tocopherol levels in seeds were observed for all cultivars and storage times. The α-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. However, the artificially aged seeds had a higher content of α-tocopherol than those naturally aged. Keywords: (Glycine max (L.) Merrill), α-tocopherol, storage. Tocoferois sao conhecidos constituintes da vitamina E, sendo os principais antioxidantes lipofilicos na soja. Eles sao substancias antioxidantes naturais e estabilizadoras, capazes de inibir a degradacao de lipidios limitando a oxidacao nao enzimatica destes compostos durante o armazenamento das sementes, a germinacao e o desenvolvimento inicial das plantulas. O objetivo deste trabalho foi determinar o conteudo de α-tocoferol nas sementes de quarto cultivares de soja envelhecidas natural e artificialmente. As sementes de quatro cultivares de soja envelhecidas em armazenamento de zero a 180 dias em camara seca (envelhecimento natural) e submetidas a altas temperaturas e umidade relativa (envelhecimento artificial) foram analisadas quanto ao teor de α-tocoferol. A quantificacao de α- tocoferol nas sementes de soja foi efetuada por cromatografia liquida de alta eficiencia (CLAE) associada ao metodo de extracao Soxhlet. Diferencas significativas nos teores de α-tocoferol nas sementes foram observadas para todas as cultivares e tempos de armazenamento. O teor de α-tocoferol das sementes de soja mostrou resposta linear crescente com o aumento dos periodos de armazenamento. Entretanto, as sementes envelhecidas artificialmente apresentaram maior teor de α-tocoferol do que as envelheci