Evaluation of Lab-on-a-Disc Technique Performance for Soil-Transmitted Helminth Diagnosis in Animals in Tanzania

Soil-transmitted helminth (STH) infections are caused by roundworms, hookworms, whipworms, and thread worms. Accurate diagnosis is essential for effective treatment, prevention, and control of these infections. This study evaluates a new diagnostic method called Single-image Parasite Quantification...

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Published in:Veterinary sciences 2024-04, Vol.11 (4), p.174
Main Authors: Rubagumya, Sarah L, Nzalawahe, Jahashi, Misinzo, Gerald, Mazigo, Humphrey D, Briet, Matthieu, Misko, Vyacheslav R, De Malsche, Wim, Legein, Filip, Justine, Nyanda C, Basinda, Namanya, Mafie, Eliakunda
Format: Article
Language:English
Subjects:
animals
cross-sectional studies
Diagnosis
diagnostic techniques
Disease transmission
Dogs
domestic pigs
Eggs
Feces
flotation
Health aspects
helminths
Households
Infections
Laboratories
LoD
McMaster
Medical research
Medicine, Experimental
Methods
Parasites
Performance evaluation
Sample size
soil-transmitted helminths
Swine
Tanzania
Trichuris
Zoonoses
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Summary:Soil-transmitted helminth (STH) infections are caused by roundworms, hookworms, whipworms, and thread worms. Accurate diagnosis is essential for effective treatment, prevention, and control of these infections. This study evaluates a new diagnostic method called Single-image Parasite Quantification (SIMPAQ), which uses a lab-on-a-disc (LoD) technique to isolate STH eggs into a single imaging zone for digital analysis. The study evaluates the purification performance of the SIMPAQ technique for detecting STH eggs in animal samples. This was a cross-sectional study conducted among 237 pigs and 281 dogs in the Morogoro region in Tanzania. Faecal samples were collected and processed with the LoD technique, as well as flotation and McMaster (McM) methods for comparison purposes. The overall prevalence of STH infections was high as per the LoD technique (74%), followed by McM (65.44%) and flotation (65.04%). Moreover, the overall performance of the LoD technique, using McM as the gold standard, was 93.51% (sensitivity), 60.89% (specificity), 81.91% (PPV), and 83.21% (NPV). The LoD technique exhibited high prevalence, sensitivity, and NPV, which demonstrates its value for STH egg detection and its crucial role in the era of accurate STH diagnosis, promoting proper management of the infection.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci11040174