Separation and identification of highly fluorescent compounds derived from trans-resveratrol in the leaves of Vitis vinifera infected by Plasmopara viticola

A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2012-03, Vol.17 (3), p.2773-2783
Main Authors: Tríska, Jan, Vrchotova, Nadezda, Olejníckova, Julie, Jílek, Rudolf, Sotolar, Radek
Format: Article
Language:English
Subjects:
2,4,6-trihydroxyphenanthrene-2-O-glucoside
Berries
Cardiovascular disease
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid - standards
Fluorescent Dyes - chemistry
Fluorescent Dyes - isolation & purification
Fungicides
Glucosides - chemistry
Glucosides - isolation & purification
Glucosides - radiation effects
Infections
Isomerism
Mass Spectrometry - standards
Peronospora
Pesticides
Phenanthrenes - chemistry
Phenanthrenes - isolation & purification
Plant Diseases - microbiology
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Leaves - chemistry
Plant Leaves - metabolism
Plant Leaves - microbiology
Plasmopara viticola
Reference Standards
Resveratrol
Stilbenes - chemistry
Stilbenes - radiation effects
trans-resveratrol
transformation
Ultraviolet Rays
Vitis - chemistry
Vitis - metabolism
Vitis - microbiology
Wineries & vineyards
Wines
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Description
Summary:A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C(18) column and gradient elution with water-acetonitrile mixtures (20-80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules17032773