Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-10, Vol.26 (21), p.6587
• Main Authors: Shang, Xiaohong, Huang, Ding, Wang, Ying, Xiao, Liang, Ming, Ruhong, Zeng, Wendan, Cao, Sheng, Lu, Liuying, Wu, Zhengdan, Yan, Huabing
• Format: Article
• Language: English
• Subjects: Amino acids; Carbohydrates; Chromatography, High Pressure Liquid; Discriminant analysis; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - metabolism; Ecological effects; Flavonoids; Herbal medicine; Isoflavones; Lipids; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medicine, Chinese Traditional; metabolite profiling; Metabolites; Metabolomics; Nonnative species; Nucleotides; Nutritive Value; Organic acids; Phenolic acids; Phenols; primary metabolites; Pueraria - chemistry; Pueraria - metabolism; Pueraria lobata; Saccharides; secondary metabolites; Tandem Mass Spectrometry; UPLC-MS/MS
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26216587

and its variety var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, . var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nutritional and medicinal values, which are caused by metabolite differences among these varieties, remains to be further clarified. Here, we performed ultra performance liquid chromatography-tandem mass spectrometry based widely targeted metabolome analysis on , var. thomsonii, and . var. montana. Among them, a total of 614 metabolites were identified, and distinguished from each other using orthogonal partial least squares discriminant analysis. Our results suggest that the nutritional differences between and its varieties can be explained by variations in the abundance of amino acids, nucleotides, saccharides, and lipids; differences in flavonoids, isoflavones, phenolic acids, organic acids, and coumarins contents caused the differences in the medicinal quality of and its varieties. Additionally, the key metabolites responsible for the classification of the three varieties were identified. This study provides new insights into the underlying metabolic causes of nutritional and medicinal variation in and its varieties.