Bacillus subtilis-Fermented Products Ameliorate the Growth Performance, Alleviate Intestinal Inflammatory Gene Expression, and Modulate Cecal Microbiota Community in Broilers during the Starter Phase under Dextran Sulfate Sodium Challenge

• Published in: The Journal of Poultry Science 2022, Vol.59(3), pp.260-271
• Main Authors: Chen, Jiun-Yu, Yu, Yu-Hsiang
• Format: Article
• Language: English
• Subjects: Bacillus subtilis; Bacteria; Biodegradation; Body weight; broiler; Cecum; Cluster analysis; Dextran; Dextran sulfate; dextran sulfate sodium; Dextrans; Diet; Dietary supplements; Fatty acids; fermented product; Gene expression; Inflammation; Intestine; Microbiota; Mucin; Poultry; Sodium; Sulfates
• ISSN: 1346-7395; 1349-0486
• DOI: 10.2141/jpsa.0210139

The aim of this study was to evaluate the effects of B. subtilis-fermented products (BSFP) on growth performance, intestinal inflammatory gene expression, and cecal microbiota community in broilers challenged with dextran sulfate sodium (DSS) in a 14-day experiment. A total of 32, 1-day-old male broiler chickens (Ross 308), were randomly divided into four groups of eight birds per group and reared individually (n＝8). The treatments consisted of a control diet without supplementation and DSS challenge, control diet plus 1.5% DSS, control diet plus 1 g/kg BSFP in combination with 1.5% DSS, and control diet plus 3 g/kg of BSFP in combination with 1.5% DSS. The results showed that BSFP supplementation (1 and 3 g/kg) partially improved body weight and average daily gain in broilers under DSS challenge. Relative to DSS treatment alone, BSFP supplementation dose-dependently increased the body weight of broilers at 7 days of age, with the average daily gain being at 1 to 7 days of age. BSFP supplementation (1 and 3 g/kg) alleviated intestinal inflammatory gene expression in broilers under DSS challenge. The richness and evenness of bacterial species in cecal digesta increased in a dose-dependent manner in the groups treated with BSFP (1 and 3 g/kg) in combination with DSS challenge, compared with the control group. Unweighted principal coordinate analysis indicated distinct clusters separating the group treated with 3 g/kg of BSFP in combination with DSS challenge from the other three groups. The abundance of short-chain fatty acid-producing bacteria (genus Ruminococcaceae_unclassified) increased and that of mucin-degrading bacteria (genus Ruminococcus torques group) decreased in the cecal digesta of broilers fed 3 g/kg of BSFP, compared with the control group. In conclusion, BSFP supplementation dose-dependently improved growth performance, reduced gut inflammation, and regulated the cecal microbiota of broilers exposed to DSS challenge during the starter phase.