Modulation of Klebsiella pneumoniae Outer Membrane Vesicle Protein Cargo under Antibiotic Treatment

is a nosocomial pathogen and an important propagator of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Like other Gram-negative bacteria, they secrete outer membrane vesicles (OMVs) that distribute virulence and resistance factors. Here, we subjected a . -XDR to subinhibitor...

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Published in:Biomedicines 2023-05, Vol.11 (6), p.1515
Main Authors: Lucena, Aline Castro Rodrigues, Ferrarini, Mariana Galvão, de Oliveira, Willian Klassen, Marcon, Bruna Hilzendeger, Morello, Luis Gustavo, Alves, Lysangela Ronalte, Faoro, Helisson
Format: Article
Language:English
Subjects:
Amikacin
antibiotic resistance
Antibiotics
Bacteria
Drug metabolism
Drug resistance
Energy metabolism
Genes
Genomes
Gram-negative bacteria
Information processing
Klebsiella pneumoniae
Liquid chromatography
Mass spectroscopy
Membrane proteins
Membrane vesicles
Meropenem
MicroRNAs
Multidrug resistance
Nanoparticles
outer membrane vesicle
Pathogenicity
Plasmids
Polymyxin B
Proteins
Proteomes
proteomics
Transmission electron microscopy
Variance analysis
Virulence
Virulence factors
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Summary:is a nosocomial pathogen and an important propagator of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Like other Gram-negative bacteria, they secrete outer membrane vesicles (OMVs) that distribute virulence and resistance factors. Here, we subjected a . -XDR to subinhibitory concentrations of meropenem, amikacin, polymyxin B, and a combination of these agents to evaluate changes in the protein cargo of OMVs through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Genome sequencing of the clinical isolate . strain HCD1 (KpHCD1) revealed the presence of 41 resistance genes and 159 virulence factors. We identified 64 proteins in KpHCD1-OMVs modulated with different antibiotic treatments involved in processing genetic information, environmental information, cell envelope formation, energy metabolism, and drug resistance. The OMV proteome expression profile suggests that OMVs may be associated with pathogenicity, survival, stress response, and resistance dissemination.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines11061515