Effects of Vitamin A on Immune Responses and Vitamin A Metabolism in Broiler Chickens Challenged with Necrotic Enteritis

• Published in: Life (Basel, Switzerland) Switzerland), 2023-05, Vol.13 (5), p.1122
• Main Authors: Guo, Shuangshuang, He, Lai, Zhang, Yuanke, Niu, Junlong, Li, Changwu, Zhang, Zhengfan, Li, Peng, Ding, Binying
• Format: Article
• Language: English
• Subjects: Aldehyde dehydrogenase; Aldehydes; Alkaline phosphatase; Antibiotics; Antibodies; Aspartate aminotransferase; Birds; broiler chicken; Broilers (Poultry); Care and treatment; Chickens; Clostridium perfringens; Creatine; Creatine kinase; Cytokines; Dehydrogenases; Deposition; Diet; Dietary supplements; Economic impact; Enteritis; Gene expression; Genes; Growth factors; Health aspects; Helper cells; Immune response; Immunomodulation; Infections; Inflammatory diseases; Interleukin 13; Janus kinase; Jejunum; Kinases; Lesions; Ligands; Liver; Lymphocytes T; Metabolism; Necrosis; necrotic enteritis; Nutrient interactions; Phospholipase C; Phosphorus; Poultry; Poultry production; Receptors; Retinene; Retinoic acid; Signal transduction; Small intestine; Spleen; Testing; Transcription factors; Transducers; Uric acid; Vaccines; Vitamin A; γ-Interferon
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life13051122

Necrotic enteritis (NE) is an important enteric inflammatory disease of poultry, and the effects of vitamin A (VitA) on NE birds are largely unknown. The present study was conducted to investigate the effects of VitA on the immune responses and VitA metabolism of NE broilers as well as the underlying mechanisms. Using a 2 × 2 factorial arrangement, 336 1-day-old Ross 308 broiler chicks were randomly assigned to 4 groups with 7 replicates. Broilers in the control (Ctrl) group were fed a basal diet without extra VitA supplementation. Broilers in the VitA group were fed a basal diet supplemented with 12,000 IU/kg of VitA. Birds in NE and VitA + NE groups were fed corresponding diets and, in addition, co-infected with spp. and on days 14 to 20. Samples of the blood, jejunum, spleen and liver were obtained on day 28 for analysis, and meanwhile, lesion scores were also recorded. The results showed that NE challenge increased lesion score in the jejunum and decreased serum glucose, total glyceride, calcium, phosphorus and uric acid levels ( < 0.05). VitA supplementation reduced the levels of serum phosphorus, uric acid and alkaline phosphatase in NE-challenged birds and increased serum low-density lipoprotein content and the activity of aspartate aminotransferase and creatine kinase ( < 0.05). Compared with the Ctrl group, the VitA and NE groups had higher mRNA expression of interferon-γ in the jejunum ( < 0.05). NE challenge up-regulated mRNA expression of interleukin ( )-13, transforming growth factor-β4, aldehyde dehydrogenase ( )-2 and in the jejunum, while VitA supplementation increased jejunal mRNA expression and hepatic VitA content, but down-regulated splenic mRNA expression ( < 0.05). The VitA + NE group had higher serum prostaglandin E levels and the Ctrl group had higher splenic mRNA expression than that of the other three groups ( < 0.05). NE challenge up-regulated jejunal retinoic acid receptor ( )-β and retinoid X receptor ( )-α as well as splenic and mRNA expression ( < 0.05). VitA supplementation up-regulated jejunal expression but down-regulated mRNA expression of , , signal transducers and activators of transcription ( ) 5 and in the spleen ( < 0.05). Moreover, compared with the Ctrl group, the VitA and NE groups had down-regulated mRNA expression of jejunal and splenic Janus kinase ( ) 1 ( < 0.05). In conclusion, NE challenge induced jejunal injury and expression of Th2 and Treg cell-related cytokines and enhanced and mRNA expression, mainly in