Effect of hyperbaric oxygen therapy on liver function during intermittent ischemia

PURPOSE: To analyze the effects of hyperbaric oxygen therapy on liver function in rats previously subjected to ischemia and reperfusion. METHODS: A randomly distribution of 23 Wistar rats was conducted into three groups: SHAM, animals subjected to surgical stress without restricting blood flow by cl...

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Published in:Acta cirúrgica brasileira 2013, Vol.28 (suppl 1), p.61-65
Main Authors: Baldim, Leticia Botigeli, Nejo Jr, Ricardo, Souza, Maria Eliza Jordani, Gomes, Maria Cecília Jordani, Picinato, Maria Aparecida Neves Cardoso, Fina, Clarice Fleury, Castro-e-Silva, Orlando
Format: Article
Language:English
Subjects:
Hyperbaric Oxygen Therapy
Liver Ischemia
Mitochondria
Reperfusion
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Summary:PURPOSE: To analyze the effects of hyperbaric oxygen therapy on liver function in rats previously subjected to ischemia and reperfusion. METHODS: A randomly distribution of 23 Wistar rats was conducted into three groups: SHAM, animals subjected to surgical stress without restricting blood flow by clamping the hepatic pedicle, IR, rats underwent hepatic vascular occlusion intermittently for two complete cycles of 15 minutes of ischemia followed by 5 min of reperfusion, IR / HBO, rats underwent hepatic pedicle clamping and thereafter exposed to hyperbaric oxygen pressure of 2 absolute atmospheres for 60 minutes. We evaluated liver function through mitochondrial function, determined by the stages 3 and 4 of respiration, respiratory control ratio (RCR) and mitochondrial permeability transition (Swelling). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were also quantified . We analyzed the results using the Mann-Whitney test and were considered significant all results with p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-86502013001300012