MTHFR 677TT is associated with decreased number of embryos and cumulative live birth rate in patients undergoing GnRHa short protocol: a retrospective study

• Published in: BMC pregnancy and childbirth 2022-03, Vol.22 (1), p.170-170, Article 170
• Main Authors: Zeng, Hong, Liu, Zefu, Zhang, Lei, Liu, Nenghui
• Format: Article
• Language: English
• Subjects: 5,10-methylenetetrahydrofolate reductase; Adult; Cumulative live birth rate; Embryo Transfer - methods; Female; Fertilization in Vitro; Genotype; Gonadotropin-Releasing Hormone - agonists; Humans; Live Birth - genetics; Methylenetetrahydrofolate Reductase (NADPH2) - genetics; Ovulation Induction - methods; Pregnancy; Pregnancy Outcome - genetics; Pregnancy Rate; Regression Analysis; Reproductive Techniques, Assisted; Retrospective analysis; Retrospective Studies
• ISSN: 1471-2393; 1471-2393
• DOI: 10.1186/s12884-022-04506-4

Whether MTHFR C677T genotype affects pregnancy outcomes following assisted reproductive technology is conflicting. And the role of MTHFR C677T genotype on cumulative live birth has not been reported. This study aims to investigate the effect of MTHFR C677T genotype on cumulative live birth following in-vitro fertilization and embryo transfer (IVF-ET). This is a retrospective cohort study that includes 1173 women undergoing their first IVF-ET. We retrospectively compared the reproductive outcomes among the groups stratified by MTHFR C677T genotypes (677CC, 677CT, 677TT). We performed interaction analysis to detect the factor that interacts with the MTHFR C677T genotype. Poisson regression analyses were used to evaluate the associations between MTHFR C677T genotypes with the number of transferable embryos and the number of good-quality embryos. Cox regression analysis was used to evaluate the association between MTHFR C677T genotypes with cumulative live birth. All regression analyses were adjusted with the confounding factors which may independently impact reproductive outcomes. There is a significant interactive effect of MTHFR 677TT genotype with GnRHa protocol on reproductive outcomes (P for interaction0.05). MTHFR 677TT genotype is associated with decreased number of transferable embryos, decreased number of good-quality embryos, and decreased cumulative live birth rate in the first complete cycle in patients undergoing GnRHa short protocol.