Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using...

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Bibliographic Details
Published in:eLife 2021-07, Vol.10
Main Authors: van der Plas, Mariena Ja, Cai, Jun, Petrlova, Jitka, Saleh, Karim, Kjellström, Sven, Schmidtchen, Artur
Format: Article
Language:English
Subjects:
bacterial infection
Biochemistry and Chemical Biology
Biomarkers
Body Fluids - metabolism
Clinical Medicine
Dermatologi och venereologi
Dermatology and Venereal Diseases
Fluids
Humans
Immunology and Inflammation
Investigations
Klinisk medicin
Mass spectrometry
Mass Spectrometry - methods
Medical and Health Sciences
Medicin och hälsovetenskap
Molecular Weight
Nosocomial infections
Peptide Fragments - analysis
peptide profiles
Peptides
peptidomics
Plasma
Proteins
Proteolysis
Proteomics
Proteomics - methods
Scientific imaging
Staphylococcal Infections - physiopathology
Staphylococcus aureus - physiology
Surgery
Ulcers
wound fluid
Wound healing
Wound Healing - physiology
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Summary:The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.66876