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Oxidative Products of Proteins and Antioxidant Potential of Thiols in Gastric Carcinoma Patients

It has been suggested that oxidative stress defined as a shift in antioxidant/oxidant balance towards oxidants is associated with the pathogenesis of many diseases, including carcinogenesis. Reactive oxygen species can induce carcinogenesis via injury to macromolecules such as DNA, carbohydrates and...

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Published in:Journal of medical biochemistry 2010-04, Vol.29 (2), p.102-106
Main Authors: Mukthapura, Anita, Shimogga, Avinash, K, Vinodchandran, Shetty, Beena, Rao, Gayathri
Format: Article
Language:English
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Summary:It has been suggested that oxidative stress defined as a shift in antioxidant/oxidant balance towards oxidants is associated with the pathogenesis of many diseases, including carcinogenesis. Reactive oxygen species can induce carcinogenesis via injury to macromolecules such as DNA, carbohydrates and proteins. Forty primary gastric carcinoma patients and 40 healthy controls were included in the study. Advanced oxidation protein products, total thiols, total protein, albumin in plasma, % hemolysis in RBC suspension and glutathione in both whole blood and plasma were estimated. Our studies demonstrated a significant increase in advanced oxidation protein products, % hemolysis (p=0.033), A:G ratio (p=0.003) and a highly significant decrease in blood glutathione (p=0.036), total thiols (p=0.001), plasma thiols other than glutathione and total antioxidant activity. The findings suggest that gastric carcinoma is associated with oxygen derived free radicals accumulation, and depletion of total antioxidant activity has lead to oxidative stress and advancement of oxidative-antioxidative disorders followed by progression of gastric cancer. Postoje podaci o tome da je oksidativni stres, koji se definiše kao promena u ravnoteži između antioksidanata i oksidanata u korist oksidanata, povezan sa patogenezom mnogih bolesti, uključujući karcinome. Reaktivne vrste kiseonika mogu izazvati nastanak karcinoma putem oštećivanja makromolekula kao što su DNK, ugljeni hidrati i proteini. Studija je obuhvatila četrdeset pacijenata sa primarnim gastričnim karcinomom i 40 zdravih osoba. Određeni su proizvodi uznapredovale oksidacije proteina, ukupni tiol, ukupni protein, albumin u plazmi, procenat hemolize u suspenziji eritrocita i glutation u punoj krvi i plazmi. Istraživanje je ukazalo na značajan porast proizvoda uznapredovale oksidacije proteina, procenta hemolize (p=0,033), odnosa A:G (p=0,033) i veoma značajan pad glutationa u krvi (p=0,036), ukupnih tiola (p=0,001), tiola u plazmi osim glutationa i ukupne antioksidantne aktivnosti. Na osnovu rezultata se može zaključiti da postoji veza između gastričnog karcinoma i akumulacije slobodnih radikala iz kiseonika i da smanjenje ukupne antioksidantne aktivnosti dovodi do oksidativnog stresa i napredovanja oksidativnih/antioksidativnih poremećaja koje prati progresija gastričnog kancera.
ISSN:1452-8258
1452-8266
DOI:10.2478/v10011-010-0013-z