Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana

• Published in: Viruses 2024-03, Vol.16 (4), p.489
• Main Authors: Wang, Xia, Yang, Jin, Hu, Haichao, Yuan, Tangyu, Zhao, Yingjie, Liu, Ying, Li, Wei, Liu, Jiaqian
• Format: Article
• Language: English
• Subjects: Arabidopsis thaliana; Bioinformatics; Chinese wheat mosaic virus; Chinese wheat mosaic virus (CWMV); Chloroplasts; Cytoplasm; Defense mechanisms; Disease resistance; Disease Resistance - genetics; eukaryotic cells; expression pattern; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genome-Wide Association Study; Genomes; Genomics; Glycosylation; glycosyltransferases; Glycosyltransferases - genetics; Glycosyltransferases - metabolism; Homeostasis; Infections; Localization; Metabolites; Nicotiana - genetics; Nicotiana - virology; Nicotiana benthamiana; Nicotiana benthamiana (N. benthamiana); Pathogens; Phylogenetics; Phylogeny; Physicochemical properties; Plant Diseases - genetics; Plant Diseases - virology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plant viruses; Potyvirus - genetics; Potyvirus - physiology; Proteins; RNA polymerase; temperature; UDP-glycosyltransferases (UGTs); Uridine; uridine diphosphate; Uridine Diphosphate - metabolism; Viruses; Wheat
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v16040489

Glycosylation, a dynamic modification prevalent in viruses and higher eukaryotes, is principally regulated by uridine diphosphate (UDP)-glycosyltransferases (UGTs) in plants. Although UGTs are involved in plant defense responses, their responses to most pathogens, especially plant viruses, remain unclear. Here, we aimed to identify UGTs in the whole genome of ( ) and to analyze their function in Chinese wheat mosaic virus (CWMV) infection. A total of 147 were identified in . To conduct a phylogenetic analysis, the UGT protein sequences of and were aligned. The gene structure and conserved motifs of the UGTs were also analyzed. Additionally, the physicochemical properties and predictable subcellular localization were examined in detail. Analysis of cis-acting elements in the putative promoter revealed that were involved in temperature, defense, and hormone responses. The expression levels of 20 containing defense-related cis-acting elements were assessed in CWMV-infected , revealing a significant upregulation of 8 . Subcellular localization analysis of three NbUGTs (NbUGT12, NbUGT16 and NbUGT17) revealed their predominant localization in the cytoplasm of leaves, and NbUGT12 was also distributed in the chloroplasts. CWMV infection did not alter the subcellular localization of NbUGT12, NbUGT16, and NbUGT17. Transient overexpression of , , and enhanced CWMV infection, whereas the knockdown of , and inhibited CWMV infection in . These could serve as potential susceptibility genes to facilitate CWMV infection. Overall, the findings throw light on the evolution and function of .