C. elegans monitor energy status via the AMPK pathway to trigger innate immune responses against bacterial pathogens

• Published in: Communications biology 2022-06, Vol.5 (1), p.643-643, Article 643
• Main Authors: Ju, Shouyong, Chen, Hanqiao, Wang, Shaoying, Lin, Jian, Ma, Yanli, Aroian, Raffi V., Peng, Donghai, Sun, Ming
• Format: Article
• Language: English
• Subjects: 14/19; 38/1; 38/22; 38/23; 38/5; 38/77; 38/89; 38/91; 42/44; 631/250/262; 631/326/421; 82/80; 82/83; 96; 96/34; 96/63; AMP-activated protein kinase; AMP-Activated Protein Kinases - metabolism; Animals; Bacillus thuringiensis; Bacillus thuringiensis - metabolism; Biology; Biomedical and Life Sciences; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Energy; Energy balance; Immune response; Immunity, Innate; Innate immunity; Intracellular; Kinases; Life Sciences; Mitochondria; Pathogens; Phosphates; Potassium; Surveillance
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-022-03589-1

Pathogen recognition and the triggering of host innate immune system are critical to understanding pathogen-host interaction. Cellular surveillance systems have been identified as an important strategy for the identification of microbial infection. In the present study, using Bacillus thuringiensis - Caenorhabditis elegans as a model, we found an approach for surveillance systems to sense pathogens. We report that Bacillus thuringiensis Cry5Ba, a typical pore-forming toxin, caused mitochondrial damage and energy imbalance by triggering potassium ion leakage, instead of directly targeting mitochondria. Interestingly, we find C. elegans can monitor intracellular energy status to trigger innate immune responses via AMP-activated protein kinase (AMPK), secreting multiple effectors to defend against pathogenic attacks. Our study indicates that the imbalance of energy status is a prevalent side effect of pathogen infection. Furthermore, the AMPK-dependent surveillance system may serve as a practicable strategy for the host to recognize and defense against pathogens. Bacillus thuringiensis toxin Cry5Ba triggers potassium ion leakage, causing mitochondrial damage and energy imbalance. C. elegans can monitor this intracellular energy imbalance via AMP-activated protein kinase to trigger innate immune responses.