Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression

• Published in: Current research in microbial sciences 2024, Vol.7, p.100256, Article 100256
• Main Authors: Shreshtha, Kumar, Raj, Satyam, Pal, Arun Kumar, Tripathi, Pooja, Choudhary, Krishna Kumar, Mitra, Debasis, Rani, Anju, Santos-Villalobos, Sergio de los, Tripathi, Vijay
• Format: Article
• Language: English
• Subjects: Biocontrol; Heavy metals; Municipal wastewater; Plant growth-promoting bacteria; Plant-associated bacteria
• ISSN: 2666-5174; 2666-5174
• DOI: 10.1016/j.crmicr.2024.100256

•Wastewater irrigation increases heavy metal-resistant bacteria in cucumber plants.•Rhizospheric and Endophytic bacteria displayed heavy metal resistance.•Rhizopheric bacteria showed high heavy metal resistance compared to endohytes.•Protius mirabilis and Bacillus cereus strains showed high PGP traits.•Protius mirabilis and Bacillus cereus showed antagonistic effect against Fusarium oxysporum f. sp. lycopersici. Wastewater contains various emerging contaminants, including heavy metals, residues of pesticides, and pharmaceuticals. Therefore, irrigation with wastewater can enhance heavy metal contamination in soil and adversely affect plant growth. To mitigate this problem, plant growth-promoting bacteria (PGPR) can improve plant growth under heavy metal stress. This study aimed to isolate and characterize rhizospheric and endophytic bacteria from the rhizosphere soil and roots of a cucumber plant irrigated with municipal wastewater. A total of 121 morphologically distinct bacterial isolates from the rhizosphere and 90 bacterial isolates from the endophytic region were isolated and tested for heavy metal resistance and in vitro plant growth-promoting characteristics, including indole-3-acetic acid (IAA) production, phosphate solubilization, Hydrogen Cyanide (HCN) production, and siderophore production. Most of the bacteria analyzed from the rhizospheric and endophytic regions showed various plant growth-promoting characteristics and were tolerant to different heavy metals at various concentrations. Bacterial strains R1 (Proteus sp.) and E2 (Bacillus sp.) were antagonistic to Fusarium oxysporum f. sp. Lycopersici. Wastewater irrigation increases heavy metal-resistant bacteria in cucumber plants, which can alleviate heavy metal stress. Additionally, Proteus sp. and Bacillus sp. isolates are potential candidates for removing heavy metal-contaminated soil and could be potential biofertilizer candidates for selected plants and biocontrol agents. [Display omitted]