Cloning and expression of SgCYP450-4 from Siraitia grosvenorii

CYP450 plays an essential role in the development and growth of the fruits of . However, little is known about the gene in . Here, based on transcriptome data, a full-length cDNA sequence of was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (...

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Bibliographic Details
Published in:Acta pharmaceutica Sinica. B 2016-11, Vol.6 (6), p.614-622
Main Authors: Tu, Dongping, Ma, Xiaojun, Zhao, Huan, Mo, Changming, Tang, Qi, Wang, Liuping, Huang, Jie, Pan, Limei
Format: Article
Language:English
Subjects:
Auxin
CYP87A3
Expression pattern
Original
Race clone
SgCYP450-4
Siraitia grosvenorii
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Summary:CYP450 plays an essential role in the development and growth of the fruits of . However, little is known about the gene in . Here, based on transcriptome data, a full-length cDNA sequence of was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) strategies. is 1677 bp in length (GenBank accession No. AEM42985.1) and contains a complete open reading frame (ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point (PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains. gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of Sg had the highest level and then decreased over time, which was consistent with the development of fruits of . Hormonal treatment could significantly induce the expression of . These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.
ISSN:2211-3835
2211-3843
DOI:10.1016/j.apsb.2016.06.009