Can Enterocin M in Combination with Sage Extract Have Beneficial Effect on Microbiota, Blood Biochemistry, Phagocytic Activity and Jejunal Morphometry in Broiler Rabbits?

• Published in: Animals (Basel) 2020-01, Vol.10 (1), p.115
• Main Authors: Pogány Simonová, Monika, Chrastinová, Ľubica, Kandričáková, Anna, Gancarčíková, Soňa, Bino, Eva, Plachá, Iveta, Ščerbová, Jana, Strompfová, Viola, Žitňan, Rudolf, Lauková, Andrea
• Format: Article
• Language: English
• Subjects: Additives; Alanine; Alanine transaminase; Animal feeding and feeds; Antibacterial activity; Bacteria; Bacteriocins; Biochemistry; Blood; Cholesterol; Coagulase; Coliforms; Consumption; Creatinine; Diet; Digestive system; Drinking water; enterocin; Experiments; Fatty acids; Feces; Feeds; Food; Food and nutrition; Gastrointestinal system; Gastrointestinal tract; High density lipoprotein; In vivo methods and tests; Laboratory animals; Lactic acid; Lactic acid bacteria; Low density lipoprotein; Microbiota; Microflora; Morphometry; Nutrition; Phagocytes; phagocytic activity; Physiological aspects; Probiotics; rabbit; Rabbits; sage; Small intestine; Spoilage; Urea; Veterinary research; Weaning
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani10010115

The present study investigated the effects of enterocin (Ent) M and sage extract alone and also in combination on the gut microflora, phagocytic activity, blood biochemistry, and morphometry of rabbits. Sixty-four rabbits (aged five weeks, M91 meatline, both sexes) were divided into three experimental groups: E (EntM; 50 µL/animal/day), S (sage; 10 µL/animal/day), and E + S (EntM + sage) groups and control group (C). The additives were administered in drinking water for a period of 21 days. Dietary supplementation of EntM and sage significantly reduced the coliforms (E: < 0.001; S: < 0.001; E + S: < 0.001) in feces, while simultaneous addition of EntM and sage decreased enterococci (E + S: < 0.0001), lactic acid bacteria (E + S: < 0.01), and coagulase-positive staphylococci (E + S: < 0.0001) in the appendix. Sage addition reduced HDL (S: < 0.001) and LDL cholesterol (S: < 0.001; E + S: < 0.001), LDL/HDL ratio (S: < 0.001; E + S: < 0.01), and increased urea (S: < 0.01; E + S: < 0.001) and creatinine (S: < 0.001; E + S: < 0.001) in serum. EntM and sage application, alone or in combination, improve the jejunal morphometry ( < 0.0001) in rabbits.