High-Intensity Low Frequency Pulsed Electromagnetic Fields Treatment Stimulates Fin Regeneration in Adult Zebrafish—A Preliminary Report

Low-Intensity electromagnetic fields (LI-PEMFs) are known to induce a trophic stimulus on bone tissue and therefore have been largely used for the treatment of several musculoskeletal disorders. High intensity (HI) PEMFs add interesting features to bio-stimulation such as electroporation, a phenomen...

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Bibliographic Details
Published in:Applied sciences 2022-08, Vol.12 (15), p.7768
Main Authors: Carnovali, Marta, Stefanetti, Noemi, Galluzzo, Alessandro, Romeo, Pietro, Mariotti, Massimo, Sansone, Valerio
Format: Article
Language:English
Subjects:
Amputation
Bone diseases
Bone growth
Bone matrix
bone regeneration
Bones
Calcein
Danio rerio
Diamagnetism
electromagnetic field
Electromagnetic fields
Electromagnetism
Electroporation
Experiments
fin
Fluorescence
Fluorescence in situ hybridization
Fluorescence microscopy
Magnetic fields
Musculoskeletal diseases
Open source software
Regeneration
Regeneration (physiology)
Signal transduction
Stimulation
Zebrafish
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Summary:Low-Intensity electromagnetic fields (LI-PEMFs) are known to induce a trophic stimulus on bone tissue and therefore have been largely used for the treatment of several musculoskeletal disorders. High intensity (HI) PEMFs add interesting features to bio-stimulation such as electroporation, a phenomenon characterized by transient increased cell permeabilization to molecules, and diamagnetism, a water-repulsive effect based on the diamagnetic properties of water and transmembrane ions gradients. Despite the rapid evolution of technology, the biological mechanisms underlying it are still poorly understood. In order to evaluate the effectiveness of this particular stimulation, HI LF-PEMFs were used to stimulate the caudal fin rays of adult zebrafish. Actually, the zebrafish fin regeneration is a simple, well understood, and widely adopted model for studying bone regeneration. A controlled amputation fin experiment was then conducted. Regenerated bone matrix of fin rays was dyed with calcein and then analysed under fluorescence microscopy. Both the length and the area of regenerated fin’s rays treated with HI LF-PEMFs resulted significantly increased when compared with non-treated.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12157768