Potential of formulated Dyadobacter jiangsuensis strain 12851 for enhanced bioremediation of chlorpyrifos contaminated soil

• Published in: Ecotoxicology and environmental safety 2021-04, Vol.213, p.112039, Article 112039
• Main Authors: Yadav, Sonal, Khan, Mohd. Aamir, Sharma, Ranju, Malik, Anushree, Sharma, Satyawati
• Format: Article
• Language: English
• Subjects: 3,5,6-trichloro-2-pyridinol; Biodegradation, Environmental; Bioremediation; Chlorpyrifos; Chlorpyrifos - metabolism; Cytophagaceae - physiology; Dyadobacter jiangsuensis; Gas chromatography-mass spectrophotometry; Soil; Soil Pollutants - metabolism; Water dispersible granules
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112039

Chlorpyrifos (O, O-diethyl O-3, 5, 6-trichloropyridin-2-yl phosphorothioate) is a toxic and chlorinated organic contaminant in soils across the globe. The present study examines the chlorpyrifos (CP) degrading potential of gram-negative bacterium Dyadobacter jiangsuensis (MTCC 12851), to be a promising and sustainable remedial approach. The proliferation of D. jiangsuensis in the chlorpyrifos spiked minimal salt media indicated the ability of this strain to utilize CP as a sole carbon source and also confirmed the utilization of 3,5,6- trichloro-2-pyridinyl (TCP) through silver nitrate assay. The strain 12851 degraded 80.36% and 76.93% chlorpyrifos (CP) in aqueous medium and soil environment, respectively. The water dispersible granules (WDG) of 45% (v/w) inoculum (bacterial suspension) were developed using talcum powder, acacia gum and alginic acid as key ingredients. The formulated strain (12851) achieved 21.13% enhanced CP degradation in soil under microcosm condition as compared to the unformulated one on 15th day of the treatment. The intermediate metabolites namely 3,5,6-trichloro-2-pyridinol (TCP), tetrahydropyridine, thiophosphate and phenol, 1, 3-bis (1,1-dimethylethyl) were detected during the CP degradation. The current investigation reveals D. jiangsuensis as a potential microbe for CP degradation and opens up the possibility of exploiting its formulations to remediate the CP polluted soils. •The potential of strain 12851 to degrade chlorpyrifos was acknowledged.•Explored the possibility of exploiting strain 12851 in formulated form.•Development of water dispersible granules of Dyadobacter jiangsuensis.•Water dispersible granules enhanced remediation of chlorpyrifos and stabilized the strain 12851 during storage period of 120 days.