Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria

Tolyporphins A-R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-5...

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Bibliographic Details
Published in:Life (Basel, Switzerland) Switzerland), 2021-07, Vol.11 (8), p.758
Main Authors: Jin, Xiaohe, Zhang, Yunlong, Zhang, Ran, Nguyen, Kathy-Uyen, Lindsey, Jonathan S, Miller, Eric S
Format: Article
Language:English
Subjects:
Bacteria
Biochemical analysis
Biosynthesis
biosynthetic gene cluster
Brasilonema
Cyanobacteria
Enzymes
Gene clusters
Genes
Genetic analysis
Genomes
Genomics
Germfree
Glucose
Gram-positive bacteria
Homology
Nostoc
Phylogenetics
Phylogeny
Pigments
Proteins
rRNA 16S
tetrapyrroles
tolyporphins
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Summary:Tolyporphins A-R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-58-2 led to identification of similar BGCs in seven other filamentous cyanobacteria, including strains sp. 106C, sp. RF31YmG, sp. FACHB-892, UFV-OR1, UFV-E1, CENA114 and sp. LEGE 06141, suggesting their potential for tolyporphins production. A similar gene cluster (BGC-2) also was identified unexpectedly in HT-58-2. Tolyporphins BGCs were not identified in unicellular cyanobacteria. Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC. Though identified with putative tolyporphins BGCs, examination of pigments extracted from three cyanobacteria has not revealed the presence of tolyporphins. Overall, the identification of BGCs and potential producers of tolyporphins presents a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles.
ISSN:2075-1729
2075-1729
DOI:10.3390/life11080758