Occurrence and risk factors of nontuberculous mycobacteria in tuberculosis-suspected patients in the north of Iran

Some Nontuberculous Mycobacteria (NTM) can occasionally infect the human population and cause infections having symptoms similar to tuberculosis (TB). This study tried to provide updated data about the frequency and diversity of NTM species. Suspicious samples of (MTB) with both positive results in...

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Bibliographic Details
Published in:Iranian journal of microbiology 2021-04, Vol.13 (2), p.190-198
Main Authors: Shafipour, Maryam, Shirzad-Aski, Hesamaddin, Ghaemi, Ezzat Allah, Sohrabi, Ahmad, Taziki, Masoumeh, Kochkaksaraei, Maya Babai, Rahimi, Somayeh
Format: Article
Language:English
Subjects:
16S rDNA
Mycobacterium simiae
Nontuberculous mycobacteria
Original
Sequence alignment
Sequence homology
Tuberculosis
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Summary:Some Nontuberculous Mycobacteria (NTM) can occasionally infect the human population and cause infections having symptoms similar to tuberculosis (TB). This study tried to provide updated data about the frequency and diversity of NTM species. Suspicious samples of (MTB) with both positive results in Ziehl-Neelsen (ZN) staining and Löwenstein-Jensen medium culturing were evaluated during January 2016 and December 2018 in Gorgan, Iran. After determination of MTB isolates by the growth rate, pigmentation status, the niacin test, and the insertion sequence 6110 (IS6110) PCR assay, other unknown isolates (presumably NTM) were detected by the rDNA sequencing method and drawing the phylogenetic tree. Based on the patients' demographic information, their risk factors were also assessed. Among 226 culture-positive samples, obtained from 2994 individuals with suspected symptoms of TB, the analyses found 12 (5.3%) NTM and three isolates. (6/12) was the most prevalent NTM species. The average nucleotide similarity value was 98.2% ± 3.7. In comparison to patients with MTB (211 confirmed cases), other mycobacterium infections were more common in patients over 65 years old (Odd ratio (95% convenience interval): 2.96 (0.69 - 12.59), P = 0.14). Although the NTM species has a small portion in TB suspected patients, their prevalence has increased, mainly in elderly patients. Moreover, was the most prevalent NTM species in our region. Therefore, identification of common species in each region is recommended and clinicians should pay more attention to them in each region.
ISSN:2008-3289
2008-4447
DOI:10.18502/ijm.v13i2.5980