Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα

• Published in: PPAR Research 2010-01, Vol.2010 (2010), p.438-455
• Main Authors: Knoch, Bianca, Barnett, Matthew P. G., Cooney, Janine, McNabb, Warren C., Barraclough, Diane, Laing, William, Zhu, Shuotun, Park, Zaneta A., MacLean, Paul, Knowles, Scott O., Roy, Nicole C.
• Format: Article
• Language: English
• Subjects: Chemical properties; Colitis; Colorectal diseases; Development and progression; Gastrointestinal diseases; Genetic aspects; Health aspects; Inflammation; Interleukins
• ISSN: 1687-4757; 1687-4765
• DOI: 10.1155/2010/621069

The interleukin-10 gene-deficient (Il10-/-) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10-/- model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10-/- mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in 12-week-old Il10-/- mice. Gene expression changes in 12-week-old Il10-/- mice were related to PPARα signaling likely to control colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined.