Protocol for preparing cyclic-phospholipid decanoate and glyceryl-didecanoate-phosphate-containing vesicles

• Published in: STAR protocols 2024-09, Vol.5 (3), p.103283, Article 103283
• Main Authors: Veena, Kollery S., Pulletikurti, Sunil, Deniz, Ashok A., Krishnamurthy, Ramanarayanan
• Format: Article
• Language: English
• Subjects: Artificial Cells - chemistry; Biophysics; Chemistry; Decanoic Acids - chemistry; Microscopy; Microscopy, Confocal - methods; Phospholipids - chemistry; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2024.103283

Cyclic-phospholipids-based vesicles can play a role in facilitating the chemical evolution of protocells from the structurally simple to the functionally more complex form. Here, we present a protocol for preparing decanoic acid-derived cyclic phospholipid and glyceryl-diester phosphate-containing vesicles. We describe steps for sample preparation, equilibration, and image acquisition using confocal microscopy. This protocol has the potential for preparing a wide variety of these phospholipid-based artificial cell constructs. For complete details on the use and execution of this protocol, please refer to Pulletikurti et al.1 [Display omitted] •Steps for preparing cyclic-phospholipid-based vesicles•Instructions for acquisition of images and analysis of vesicles from the samples•Troubleshooting steps and guidance on sample preparation and equilibration Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cyclic-phospholipids-based vesicles can play a role in facilitating the chemical evolution of protocells from the structurally simple to the functionally more complex form. Here, we present a protocol for preparing decanoic acid-derived cyclic phospholipid and glyceryl-diester phosphate-containing vesicles. We describe steps for sample preparation, equilibration, and image acquisition using confocal microscopy. This protocol has the potential for preparing a wide variety of these phospholipid-based artificial cell constructs.