COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performan...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 2016-01, Vol.58 (1), p.5-4
Main Authors: Menezes, Camila Braz, Mello, Mariana dos Santos, Tasca, Tiana
Format: Article
Language:English
Subjects:
Culture Media
Diagnosis
Female
Fixing and staining techniques
Humans
Morphology
Original
Sensitivity and Specificity
Staining and Labeling - methods
Trichomonas Infections - diagnosis
Trichomonas vaginalis
Trichomonas vaginalis - isolation & purification
Trichomonas vaginalis - microbiology
TROPICAL MEDICINE
Vaginal Smears
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Summary:Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. The smears were fixed and submitted to different methods of permanent staining and then, the morphological aspects of the parasites were analyzed and compared. The Papanicolaou staining with ethanol as the fixative solution showed to be the best method of permanent staining. Our data suggest that staining techniques in association with wet mount examination of fresh specimens contribute to increase the sensitivity in the diagnosis of trichomoniasis.
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/S1678-994620160005