Simultaneous Quantification of Eight Marker Components in Traditional Herbal Formula, Haepyoyijin-Tang Using HPLC–PDA

Haepyoyijin-tang (HPYJT) is a traditional herbal prescription that is composed of 12 medicinal herbs. Although HPYJT is frequently used in patients with asthma in Korea, no quality assessment protocols have been developed. In the present study, qualitative and quantitative analyses were performed us...

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Bibliographic Details
Published in:Applied sciences 2020-06, Vol.10 (11), p.3888
Main Authors: Seo, Chang-Seob, Lee, Mee-Young
Format: Article
Language:English
Subjects:
Accuracy
Acids
Analysis
Chromatography
Composition
Flavonoids
Gene expression
Haepyoyijin-tang
Herbal medicine
Herbals
Hesperidin
High performance liquid chromatography
HPLC–PDA
Identification and classification
Linearity
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Measurement
Medicinal herbs
Medicine
Medicine, Botanic
Medicine, Herbal
Phytochemicals
Qualitative analysis
Quality assessment
Quality control
Quantitative analysis
Retention
Rosmarinic acid
simultaneous quantification
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Summary:Haepyoyijin-tang (HPYJT) is a traditional herbal prescription that is composed of 12 medicinal herbs. Although HPYJT is frequently used in patients with asthma in Korea, no quality assessment protocols have been developed. In the present study, qualitative and quantitative analyses were performed using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry on the eight main components (mulberroside A, amygalin, liquiritin apioside, liquiritin, narirutin, hesperidin, rosmarinic acid, and glycyrrhizinic acid) to establish a quality control protocol for HPYJT. The simultaneous analysis method developed was satisfactorily validated with respect to linearity, limit of detection, limit of quantification, recovery, and precision. This analytical method thus provides an efficient approach for the evaluation of HPYJT quality.
ISSN:2076-3417
2076-3417
DOI:10.3390/app10113888