Region-resolved multi-omics of the mouse eye

• Published in: Cell reports (Cambridge) 2023-02, Vol.42 (2), p.112121-112121, Article 112121
• Main Authors: Xiang, Hang, Zhang, Bohan, Wang, Yunzhi, Xu, Ning, Zhang, Fan, Luo, Rongkui, Ji, Minbiao, Ding, Chen
• Format: Article
• Language: English
• Subjects: CP: Cell biology; lipidome; MALDI-TOF imaging; mouse eye; multimodal multiphoton; region-resolved proteome
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2023.112121

The eye is a complex organ consisting of multiple compartments with unique and specialized properties, and small disturbances in one eye region can result in impaired vision and blindness. Although there have been advancements in ocular research, the hierarchical molecular network in region-wide resolution, indicating the division of labor and crosstalk among different eye regions, is not yet comprehensively illuminated. Here, we present an atlas of region-resolved proteome and lipidome of mouse eye. Multiphoton microscopy-guided laser microdissection combined with in-depth label-free proteomics identifies 13,536 proteins across various mouse eye regions. Further integrative analysis of spectral imaging, label-free proteome, and imaging mass spectrometry of the lipidome and phosphoproteome reveals distinctive molecular features, including proteins and lipids of various anatomical mouse eye regions. These deposited datasets and our open proteome server integrating all information provide a valuable resource for future functional and mechanistic studies of mouse eye and ocular disease. [Display omitted] •Region-resolved multi-omics deciphers the complex molecular features of the mouse eye•Multiphoton imaging provides aerial view of the distribution of molecules•Proteomics analysis reveals the distinguished pathways functioning in each region•Region-specific protein-lipid connections are identified in the four mouse eye regions The eye is a complex organ, which consists of multiple compartments with unique and specialized properties that reflect their varied functions. Xiang et al. present a region-resolved mouse eye proteome and lipidome that provides a physiological reference map that may serve as a resource for the public in the future.