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Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the prese...
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Published in: | Memórias do Instituto Oswaldo Cruz 2010-11, Vol.105 (7), p.904-907 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Paracoccidioidomycosis should be differentiated from other
opportunistic diseases in human immunodeficiency virus (HIV)/acquired
immunodeficiency syndrome (AIDS) patients who live in Latin America.
Laboratory investigation can begin with serological tests, which are
rapid and efficient. In the present study, double immunodiffusion
(DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked
immunosorbent assay (ELISA) tests were assessed for the detection of
anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected
with HIV. The results were compared to those obtained for 75
non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P.
brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95%
(ELISA) of the patients with HIV/AIDS, significantly lower rates than
those detected in cases of endemic paracoccidioidomycosis, which were
89%, 99% and 100%, respectively. The reactive sera of HIV-infected
patients also showed lower anti-P. brasiliensis antibody titres than
those of non-HIV-infected patients. Despite the lower intensity of the
specific humoral response, serological tests are useful for the
diagnosis of opportunistic paracoccidioidomycosis in the HIV/AIDS
population. We suggest optimization of the laboratory diagnosis by
combining the ELISA test with CIEP or DID. |
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ISSN: | 1678-8060 0074-0276 1678-8060 0074-0276 |
DOI: | 10.1590/S0074-02762010000700011 |