Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology

Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression ( OsRUS1-OX ) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was...

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Bibliographic Details
Published in:Scientific reports 2022-04, Vol.12 (1), p.6736-6736, Article 6736
Main Authors: Yu, Ning, Liang, Yaping, Wang, Qingping, Peng, Xinxiang, He, Zhenghui, Hou, Xuewen
Format: Article
Language:English
Subjects:
631/337
631/449
Gene Expression Profiling
Gene Expression Regulation, Plant
Genotype & phenotype
Humanities and Social Sciences
Leaves
multidisciplinary
Oryza - genetics
Oryza - metabolism
Phenotypes
Plant hormones
Plant Leaves - metabolism
Rice
Science
Science (multidisciplinary)
Signal transduction
Sunlight
Transcriptome
Transcriptomics
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Summary:Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression ( OsRUS1-OX ) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was utilized to identify differentially expressed genes between flag leaves of OsRUS1-OX and wildtype under sunlight. 2920 genes were differentially expressed between OsRUS1-OX and WT, of which 1660 upregulated and 1260 downregulated. Six of the 16 genes in GO: 0009415 (response to water stimulus) were significantly upregulated in OsRUS1-OX . The differentially expressed genes between WT and OsRUS1-OX were assigned to 110 KEGG pathways. 42 of the 222 genes in KEGG pathway dosa04075 (Plant hormone signal transduction) were differentially expressed between WT and OsRUS1-OX . The identified genes in GO:0009415 and KEGG pathway dosa04075 were good candidates to explain the leaf rolling phenotype of OsRUS1-OX . The expression patterns of the 15 genes identified by RNA-Seq were verified by qRT-PCR. Based on transcriptomic and qRT-PCR analysis, a mechanism for the leaf rolling phenotype of OsRUS1-OX was proposed. The differential expression profiles between WT and OsRUS1-OX established by this study provide important insights into the molecular mechanism behind the leaf rolling phenotype of OsRUS1-OX .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-10784-x