Human Cord Blood-Derived Mesenchymal Stem Cells Home and Survive in the Marrow of Immunodeficient Mice after Systemic Infusion

Bone marrow is the residence site of mesenchymal stem cells (MSC), which upon commitment and maturation develop into several mesenchymal phenotypes. Recently, we have described the presence of MSC in human cord blood (cbMSC) and informed that their properties are the same as those for MSC obtained f...

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Bibliographic Details
Published in:Cell transplantation 2003-09, Vol.12 (6), p.555-561
Main Authors: Erices, Alejandro A., Allers, Carolina I., Conget, Paulette A., Rojas, Cecilia V., Minguell, José J.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow - immunology
Bone Marrow - surgery
Cell Differentiation - immunology
Cell Lineage - immunology
Cell Size - immunology
Chemotaxis - immunology
Cord Blood Stem Cell Transplantation - methods
Cord Blood Stem Cell Transplantation - trends
Disease Models, Animal
DNA - metabolism
Female
Fetal Blood - cytology
Globins - genetics
Graft Survival - immunology
Humans
Immunologic Deficiency Syndromes - immunology
Immunologic Deficiency Syndromes - therapy
Immunophenotyping
Infant, Newborn
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stem Cell Transplantation - trends
Mice
Mice, Nude
Stromal Cells - cytology
Stromal Cells - immunology
Transplantation, Heterologous
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Summary:Bone marrow is the residence site of mesenchymal stem cells (MSC), which upon commitment and maturation develop into several mesenchymal phenotypes. Recently, we have described the presence of MSC in human cord blood (cbMSC) and informed that their properties are the same as those for MSC obtained from adult bone marrow. In this study we have investigated the capability of transplanted cbMSC to home and survive in the marrow of unconditioned nude mice. cbMSC utilized for transplantation studies were characterized by morphology, differentiation potential, and immunophenotype. After transplantation by systemic infusion, human DNA (as detected by PCR amplification of human-specific β-globin gene) was detected in the marrow of recipients as well as in ex vivo-expanded stromal cells prepared from the marrow of transplanted animals. These results demonstrate homing and survival of cbMSC into the recipient marrow and also suggest a mesenchymal-orientated fate of engrafted cells, because human DNA was also detected in cells of other recipient tissues, like cardiac muscle, teeth, and spleen.
ISSN:0963-6897
1555-3892
DOI:10.3727/000000003108747154