Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy

Osteosarcoma (OSA) is a rare cancer both in human and dog although the incidence rate in dogs is 27 times higher than in human. Many studies employed D-17 as cell line for in vitro test to evaluate conventional anticancer therapies; however, little is known about D-17 cell line. The aim of our study...

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Bibliographic Details
Published in:Animals (Basel) 2020-11, Vol.10 (11), p.1981
Main Authors: Modesto, Paola, Castrillo Fernandez, Jordi Leonardo, Martini, Isabella, Zoccola, Roberto, Pugliano, Maria Concetta, De Ciucis, Chiara Grazia, Goria, Maria, Ferrari, Angelo, Razzuoli, Elisabetta
Format: Article
Language:English
Subjects:
Biomedical materials
Bone cancer
Bone tumors
Cancer
Care and treatment
CD14 antigen
Cell cycle
Cell lines
cytokines
D-17
Dogs
Evaluation
Gene expression
Genes
Immune response
Immune system
In vitro methods and tests
Inflammation
Inflammatory response
Innate immunity
Interleukin 1
Interleukin 10
Interleukin 15
Interleukin 18
Medical prognosis
Metastases
Microorganisms
Nitric-oxide synthase
Osteosarcoma
Physiological aspects
PTEN protein
Reagents
Salmonella
Salmonella typhimurium
Software
TLR5 protein
Toll-like receptors
Tumors
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Summary:Osteosarcoma (OSA) is a rare cancer both in human and dog although the incidence rate in dogs is 27 times higher than in human. Many studies employed D-17 as cell line for in vitro test to evaluate conventional anticancer therapies; however, little is known about D-17 cell line. The aim of our study was to evaluate the basal level of gene expression of pivotal molecules in the innate immune response and cell cycle regulation and to establish the ability of this cell line to react to Salmonella typhimurium (ST) infective stressor. IL15, IL10, iNOS, TLR5, CD14, PTEN and IL18 were expressed in an inconsistent manner among experiments. The other genes under study were expressed in all samples. ST showed ability to penetrate D-17 causing pro-inflammatory response. Our results outline the expression in D-17 of important genes involved in innate immune response. These results provide important data on D-17 basal gene expression profile useful for in vitro preliminary evaluation of new therapeutic approaches.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani10111981