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Specific Urinary Metabolites in Malignant Melanoma
Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine. Urine samples of 133 individuals (82 malignant melan...
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| Published in: | Medicina (Kaunas, Lithuania) Lithuania), 2019-05, Vol.55 (5), p.145 |
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| creator | Valko-Rokytovská, Marcela Hubková, Beáta Birková, Anna Mašlanková, Jana Stupák, Marek Zábavníková, Marianna Čižmárová, Beáta Mareková, Mária |
| description | Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine.
Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically.
Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively).
HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer. |
| doi_str_mv | 10.3390/medicina55050145 |
| format | article |
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Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically.
Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively).
HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer.</description><identifier>ISSN: 1648-9144</identifier><identifier>ISSN: 1010-660X</identifier><identifier>EISSN: 1648-9144</identifier><identifier>EISSN: 1010-660X</identifier><identifier>DOI: 10.3390/medicina55050145</identifier><identifier>PMID: 31100919</identifier><language>eng</language><publisher>Switzerland: MDPI</publisher><subject>5,6-dihydroxyindole-2-carboxylic acid ; 5-hydroxyindole-3-acetic acid ; Adult ; Aged ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - urine ; Female ; homovanilic acid ; Homovanillic Acid - analysis ; Homovanillic Acid - urine ; HPLC ; Humans ; Hydroxyindoleacetic Acid - analysis ; Hydroxyindoleacetic Acid - urine ; Indican - analysis ; Indican - urine ; Indoles - analysis ; Indoles - urine ; indoxyl sulphate ; Male ; malignant melanoma ; Melanoma - physiopathology ; Melanoma - urine ; metabolite ; Middle Aged ; tryptophan ; Tryptophan - analysis ; Tryptophan - urine ; urine ; vanilmandelic acid ; Vanilmandelic Acid - analysis ; Vanilmandelic Acid - urine</subject><ispartof>Medicina (Kaunas, Lithuania), 2019-05, Vol.55 (5), p.145</ispartof><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-af5fe0d01c37e7c2a0df8f3894a1c24cd21ba3c5b02db04e2c42a209868fa1443</citedby><cites>FETCH-LOGICAL-c528t-af5fe0d01c37e7c2a0df8f3894a1c24cd21ba3c5b02db04e2c42a209868fa1443</cites><orcidid>0000-0002-6291-4014 ; 0000-0002-2786-0620 ; 0000-0002-5074-0125</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571597/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571597/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,37013,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31100919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valko-Rokytovská, Marcela</creatorcontrib><creatorcontrib>Hubková, Beáta</creatorcontrib><creatorcontrib>Birková, Anna</creatorcontrib><creatorcontrib>Mašlanková, Jana</creatorcontrib><creatorcontrib>Stupák, Marek</creatorcontrib><creatorcontrib>Zábavníková, Marianna</creatorcontrib><creatorcontrib>Čižmárová, Beáta</creatorcontrib><creatorcontrib>Mareková, Mária</creatorcontrib><title>Specific Urinary Metabolites in Malignant Melanoma</title><title>Medicina (Kaunas, Lithuania)</title><addtitle>Medicina (Kaunas)</addtitle><description>Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine.
Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically.
Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively).
HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer.</description><subject>5,6-dihydroxyindole-2-carboxylic acid</subject><subject>5-hydroxyindole-3-acetic acid</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - urine</subject><subject>Female</subject><subject>homovanilic acid</subject><subject>Homovanillic Acid - analysis</subject><subject>Homovanillic Acid - urine</subject><subject>HPLC</subject><subject>Humans</subject><subject>Hydroxyindoleacetic Acid - analysis</subject><subject>Hydroxyindoleacetic Acid - urine</subject><subject>Indican - analysis</subject><subject>Indican - urine</subject><subject>Indoles - analysis</subject><subject>Indoles - urine</subject><subject>indoxyl sulphate</subject><subject>Male</subject><subject>malignant melanoma</subject><subject>Melanoma - physiopathology</subject><subject>Melanoma - urine</subject><subject>metabolite</subject><subject>Middle Aged</subject><subject>tryptophan</subject><subject>Tryptophan - analysis</subject><subject>Tryptophan - urine</subject><subject>urine</subject><subject>vanilmandelic acid</subject><subject>Vanilmandelic Acid - analysis</subject><subject>Vanilmandelic Acid - urine</subject><issn>1648-9144</issn><issn>1010-660X</issn><issn>1648-9144</issn><issn>1010-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpdkctLAzEQxoMovu-epEcv1clrN3sRRHxBxYP2HGbzqJHtpiZbwf_eaKuopxnm8ZuP-Qg5onDKeQNnc2eDCT1KCRKokBtkl1ZCjRsqxOavfIfs5fwCwJms2TbZ4ZQCNLTZJexx4UzwwYymqYDS--jeDdjGLgwuj0I_uscuzHrsh9LosI9zPCBbHrvsDtdxn0yvr54ub8eTh5u7y4vJ2EimhjF66R1YoIbXrjYMwXrluWoEUsOEsYy2yI1sgdkWhGNGMGTQqEp5LJr5PrlbcW3EF71IYV7k6YhBfxVimmlMQzCd0142ta183RpJhXWtEpWUVWsrShUoRgvrfMVaLNvyNOP6IWH3B_q304dnPYtvupI1LfACOFkDUnxdujzoecjGdeUlLi6zZowzkEIJVUZhNWpSzDk5_3OGgv60Tf-3rawc_5b3s_DtE_8ADe2U8w</recordid><startdate>20190516</startdate><enddate>20190516</enddate><creator>Valko-Rokytovská, Marcela</creator><creator>Hubková, Beáta</creator><creator>Birková, Anna</creator><creator>Mašlanková, Jana</creator><creator>Stupák, Marek</creator><creator>Zábavníková, Marianna</creator><creator>Čižmárová, Beáta</creator><creator>Mareková, Mária</creator><general>MDPI</general><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6291-4014</orcidid><orcidid>https://orcid.org/0000-0002-2786-0620</orcidid><orcidid>https://orcid.org/0000-0002-5074-0125</orcidid></search><sort><creationdate>20190516</creationdate><title>Specific Urinary Metabolites in Malignant Melanoma</title><author>Valko-Rokytovská, Marcela ; Hubková, Beáta ; Birková, Anna ; Mašlanková, Jana ; Stupák, Marek ; Zábavníková, Marianna ; Čižmárová, Beáta ; Mareková, Mária</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-af5fe0d01c37e7c2a0df8f3894a1c24cd21ba3c5b02db04e2c42a209868fa1443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>5,6-dihydroxyindole-2-carboxylic acid</topic><topic>5-hydroxyindole-3-acetic acid</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biomarkers, Tumor - urine</topic><topic>Female</topic><topic>homovanilic acid</topic><topic>Homovanillic Acid - analysis</topic><topic>Homovanillic Acid - urine</topic><topic>HPLC</topic><topic>Humans</topic><topic>Hydroxyindoleacetic Acid - analysis</topic><topic>Hydroxyindoleacetic Acid - urine</topic><topic>Indican - analysis</topic><topic>Indican - urine</topic><topic>Indoles - analysis</topic><topic>Indoles - urine</topic><topic>indoxyl sulphate</topic><topic>Male</topic><topic>malignant melanoma</topic><topic>Melanoma - physiopathology</topic><topic>Melanoma - urine</topic><topic>metabolite</topic><topic>Middle Aged</topic><topic>tryptophan</topic><topic>Tryptophan - analysis</topic><topic>Tryptophan - urine</topic><topic>urine</topic><topic>vanilmandelic acid</topic><topic>Vanilmandelic Acid - analysis</topic><topic>Vanilmandelic Acid - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valko-Rokytovská, Marcela</creatorcontrib><creatorcontrib>Hubková, Beáta</creatorcontrib><creatorcontrib>Birková, Anna</creatorcontrib><creatorcontrib>Mašlanková, Jana</creatorcontrib><creatorcontrib>Stupák, Marek</creatorcontrib><creatorcontrib>Zábavníková, Marianna</creatorcontrib><creatorcontrib>Čižmárová, Beáta</creatorcontrib><creatorcontrib>Mareková, Mária</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Medicina (Kaunas, Lithuania)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valko-Rokytovská, Marcela</au><au>Hubková, Beáta</au><au>Birková, Anna</au><au>Mašlanková, Jana</au><au>Stupák, Marek</au><au>Zábavníková, Marianna</au><au>Čižmárová, Beáta</au><au>Mareková, Mária</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific Urinary Metabolites in Malignant Melanoma</atitle><jtitle>Medicina (Kaunas, Lithuania)</jtitle><addtitle>Medicina (Kaunas)</addtitle><date>2019-05-16</date><risdate>2019</risdate><volume>55</volume><issue>5</issue><spage>145</spage><pages>145-</pages><issn>1648-9144</issn><issn>1010-660X</issn><eissn>1648-9144</eissn><eissn>1010-660X</eissn><abstract>Melanin, which has a confirmed role in melanoma cell behaviour, is formed in the process of melanogenesis and is synthesized from tryptophan, L-tyrosine and their metabolites. All these metabolites are easily detectable by chromatography in urine.
Urine samples of 133 individuals (82 malignant melanoma patients and 51 healthy controls) were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). The diagnosis of malignant melanoma was confirmed histologically.
Chromatograms of melanoma patients showed increased levels of 5,6-dihydroxyindole-2-carboxylic acid, vanilmandelic acid, homovanilic acid, tryptophan, 5-hydroxyindole-3-acetic acid, and indoxyl sulphate compared to healthy controls. Concentration of indoxyl sulphate, homovanilic acid and tryptophan were significantly increased even in the low clinical stage 0 of the disease (indoxyl sulphate, homovanilic acid and tryptophan in patients with clinical stage 0 vs. controls expressed as medium/ interquartile range in µmol/mmol creatinine: 28.37/15.30 vs. 5.00/6.91; 47.97/33.08 vs. 7.33/21.25; and 16.38/15.98 vs. 3.46/6.22, respectively).
HPLC detection of metabolites of L-tyrosine and tryptophan in the urine of melanoma patients may play a significant role in diagnostics as well as a therapeutic strategy of melanoma cancer.</abstract><cop>Switzerland</cop><pub>MDPI</pub><pmid>31100919</pmid><doi>10.3390/medicina55050145</doi><orcidid>https://orcid.org/0000-0002-6291-4014</orcidid><orcidid>https://orcid.org/0000-0002-2786-0620</orcidid><orcidid>https://orcid.org/0000-0002-5074-0125</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Medicina (Kaunas, Lithuania), 2019-05, Vol.55 (5), p.145 |
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| language | eng |
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| subjects | 5,6-dihydroxyindole-2-carboxylic acid 5-hydroxyindole-3-acetic acid Adult Aged Biomarkers, Tumor - analysis Biomarkers, Tumor - urine Female homovanilic acid Homovanillic Acid - analysis Homovanillic Acid - urine HPLC Humans Hydroxyindoleacetic Acid - analysis Hydroxyindoleacetic Acid - urine Indican - analysis Indican - urine Indoles - analysis Indoles - urine indoxyl sulphate Male malignant melanoma Melanoma - physiopathology Melanoma - urine metabolite Middle Aged tryptophan Tryptophan - analysis Tryptophan - urine urine vanilmandelic acid Vanilmandelic Acid - analysis Vanilmandelic Acid - urine |
| title | Specific Urinary Metabolites in Malignant Melanoma |
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