Effects of Deoxynivalenol and Fumonisins Fed in Combination to Beef Cattle: Immunotoxicity and Gene Expression

• Published in: Toxins 2021-10, Vol.13 (10), p.714
• Main Authors: Roberts, Heaven L, Bionaz, Massimo, Jiang, Duo, Doupovec, Barbara, Faas, Johannes, Estill, Charles T, Schatzmayr, Dian, Duringer, Jennifer M
• Format: Article
• Language: English
• Subjects: Animal Feed - adverse effects; Animals; Beef; Beef cattle; Blood; Cattle; Cattle - genetics; Cattle - immunology; Cattle - metabolism; CD4 antigen; CD4-Positive T-Lymphocytes; CD8 antigen; CD8-Positive T-Lymphocytes; Cell membranes; Cellular structure; Cytotoxicity; Data processing; Deoxynivalenol; Diet; Diet - veterinary; Environmental information; Evaluation; Feeds; Food Contamination; fumonisin; Fumonisins; Fumonisins - toxicity; Fusarium; Gene expression; Gene Expression Regulation - drug effects; Gene sequencing; immune function; Immune status; Immune system; Immune System - drug effects; Immunotoxicity; Impact analysis; Infectious diseases; Information processing; Leukocytes; Leukocytes (neutrophilic); Livestock; Lymphocytes; Lymphocytes T; Male; Membrane structure; Membrane structures; Metabolic disorders; Metabolites; Monocytes; Mycotoxins; Neutrophils; Peripheral blood; RNA-Seq; Signaling; Toxicity; Toxins; Transcription; Trichothecenes - toxicity
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins13100714

We evaluated the effects of a treatment diet contaminated with 1.7 mg deoxynivalenol and 3.5 mg fumonisins (B1, B2 and B3) per kg ration on immune status and peripheral blood gene expression profiles in finishing-stage Angus steers. The mycotoxin treatment diet was fed for a period of 21 days followed by a two-week washout period during which time all animals consumed the control diet. Whole-blood leukocyte differentials were performed weekly throughout the experimental and washout period. Comparative profiles of CD4 and CD8 T cells, along with bactericidal capacity of circulating neutrophils and monocytes were evaluated at 0, 7, 14, 21 and 35 days. Peripheral blood gene expression was measured at 0, 7, 21 and 35 days via RNA sequencing. Significant increases in the percentage of CD4 CD8 T cells were observed in treatment-fed steers after two weeks of treatment and were associated with decreased CD4:CD8 T-cell ratios at this same timepoint ( ≤ 0.10). No significant differences were observed as an effect of treatment in terms of bactericidal capacity at any timepoint. Dietary treatments induced major changes in transcripts associated with endocrine, metabolic and infectious diseases; protein digestion and absorption; and environmental information processing (inhibition of signaling and processing), as evaluated by dynamic impact analysis. DAVID analysis also suggested treatment effects on oxygen transport, extra-cellular signaling, cell membrane structure and immune system function. These results indicate that finishing-stage beef cattle are susceptible to the immunotoxic and transcript-inhibitory effects of deoxynivalenol and fumonisins at levels which may be realistically encountered in feedlot situations.