Conserved Influenza Hemagglutinin, Neuraminidase and Matrix Peptides Adjuvanted with ALFQ Induce Broadly Neutralizing Antibodies

A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza...

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Bibliographic Details
Published in:Vaccines (Basel) 2021-06, Vol.9 (7), p.698
Main Authors: Sei, Clara J., Rao, Mangala, Schuman, Richard F., Daum, Luke T., Matyas, Gary R., Rikhi, Nimisha, Muema, Kevin, Anderson, Alexander, Jobe, Ousman, Kroscher, Kellie A., Alving, Carl R., Fischer, Gerald W.
Format: Article
Language:English
Subjects:
ALFQ
Amino acids
Antibodies
Antigens
Binding sites
Clinical trials
Development strategies
Epitopes
Exo-a-sialidase
Genomes
Hemagglutinins
Heroin
Immune response (cell-mediated)
Immune response (humoral)
Immune system
Immunization
Immunogenicity
Immunoglobulin G
Influenza
Isotypes
liposomes
Mutation
Neutralizing
Pandemics
Peptides
Proteins
QS21 (also known as QS-21)
Tetanus
universal vaccine
Vaccines
Viruses
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Summary:A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza strains. The unconjugated and CRM-conjugated composite peptides formulated with ALFQ were highly immunogenic and induced both humoral and cellular immune responses in mice. Broadly reactive serum antibodies were induced across various IgG isotypes. Mice immunized with the unconjugated composite peptide developed antibody responses earlier than mice immunized with conjugated peptides, and the IgG antibodies were broadly reactive and neutralizing across Groups 1 and 2 influenza viruses. Multi-epitope unconjugated influenza composite peptides formulated with ALFQ provide a novel strategy for the development of a universal influenza vaccine. These synthetic peptide vaccines avoid the pitfalls of egg-produced influenza vaccines and production can be scaled up rapidly and economically.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines9070698