Loading…
First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China
Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the preval...
Saved in:
| Published in: | Parasites & vectors 2021-01, Vol.14 (1), p.11-11, Article 11 |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3 |
| container_end_page | 11 |
| container_issue | 1 |
| container_start_page | 11 |
| container_title | Parasites & vectors |
| container_volume | 14 |
| creator | Deng, Lei Yao, Jingxin Chen, Shanyu He, Tingmei Chai, Yijun Zhou, Ziyao Shi, Xiaogang Liu, Haifeng Zhong, Zhijun Fu, Hualin Peng, Guangneng |
| description | Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China.
A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis.
Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P |
| doi_str_mv | 10.1186/s13071-020-04515-2 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_f5ad19d820c34984bd5d28a5aeb0ed43</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A650590972</galeid><doaj_id>oai_doaj_org_article_f5ad19d820c34984bd5d28a5aeb0ed43</doaj_id><sourcerecordid>A650590972</sourcerecordid><originalsourceid>FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3</originalsourceid><addsrcrecordid>eNqFkluLEzEcxQdR3HX1C_ggA77ow9TcLy_CWlwtLAhenkOaS5tlmtQko9ZPb7pd160IkodJ_vmdQ-Zwuu4pBDMIBXtVIAYcDgCBARAK6YDudaeQUzZgDOj9O_uT7lEpVwAwICl72J1gTAAXUJx27iLkUvtgXazBB6NrSLHX0fabNDozjTr3ZVrW3TbEVZ98_2bUpSazKzWUvmxnfYj9z5SaJGz0WPbHkqa6_u5KdTn283WI-nH3wLdL9-Tme9Z9uXj7ef5-uPzwbjE_vxwMw6AOyHuCGfFEO24tQJpj74ggzhErKJZYCMCpZhB40SbISQHY0jpgrUeWO3zWLQ6-Nukrtc3tSXmnkg7qepDySulcgxmd8lRbKJsJMJhIQZaWWiQ01W4JnCW4eb0-eG2n5cZZ0wLKejwyPb6JYa1W6ZviXAgJRDN4cWOQ09epxaE2oRg3jjq6NBWFKCKISUrJ_1HCGURSSt7Q53-hV2nKsaW6p4SgnAH6h1rp9q8h-tSeaPam6pxRQCWQHDVq9g-qLes2waTofGjzI8HLI0FjqvtRV3oqRS0-fTxm0YE1OZWSnb-NDgK176869Fe1_qrr_qq96Nnd0G8lvwuLfwGvKeoy</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2478857605</pqid></control><display><type>article</type><title>First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Deng, Lei ; Yao, Jingxin ; Chen, Shanyu ; He, Tingmei ; Chai, Yijun ; Zhou, Ziyao ; Shi, Xiaogang ; Liu, Haifeng ; Zhong, Zhijun ; Fu, Hualin ; Peng, Guangneng</creator><creatorcontrib>Deng, Lei ; Yao, Jingxin ; Chen, Shanyu ; He, Tingmei ; Chai, Yijun ; Zhou, Ziyao ; Shi, Xiaogang ; Liu, Haifeng ; Zhong, Zhijun ; Fu, Hualin ; Peng, Guangneng</creatorcontrib><description>Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China.
A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis.
Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P < 0.05). There are also significant differences in the prevalence of Blastocystis sp. among different species (P < 0.05), and the highest of Blastocystis sp. prevalence was observed in white-cheeked gibbon, black great squirrel, and red giant flying squirrel (100%). Subtype analysis of Blastocystis sp. revealed nine subtypes, including six zoonotic STs (ST1-5, and ST8) and three animal-specific STs (ST10, ST14, and ST17), with ST17 as the predominant subtype (26/66) in Blastocystis sp.-positive isolates.
To our knowledge, this is the first report on the prevalence and subtype distribution of Blastocystis sp. among captive wildlife in zoos in southwestern China. This study highlights that these animals may serve as reservoirs for human Blastocystis sp. infections.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/s13071-020-04515-2</identifier><identifier>PMID: 33407818</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Animals, Wild - parasitology ; Animals, Zoo - parasitology ; Blastocystis ; Blastocystis - genetics ; Blastocystis - isolation & purification ; Blastocystis Infections - epidemiology ; Blastocystis sp ; captive animals ; Captive wildlife ; China ; China - epidemiology ; Deoxyribonucleic acid ; digestive tract ; Disease Reservoirs ; Distribution ; DNA ; DNA, Protozoan - genetics ; DNA, Ribosomal - genetics ; Feces ; Feces - parasitology ; Flight ; Gastrointestinal system ; Gastrointestinal tract ; genes ; Genetic aspects ; Genetic Variation ; Genotype ; Health aspects ; Host-parasite relationships ; human diseases ; Humans ; Identification and classification ; Infections ; Irritable bowel syndrome ; Microbiota ; Nucleotide sequence ; Parasites ; Pathogenicity ; Pathogens ; PCR ; Phylogenetic analysis ; Phylogenetics ; Phylogeny ; Prevalence ; Protozoa ; rRNA ; Species ; Squirrels ; Subtypes ; Wildlife ; Zoo animals ; Zoonoses ; Zoos</subject><ispartof>Parasites & vectors, 2021-01, Vol.14 (1), p.11-11, Article 11</ispartof><rights>COPYRIGHT 2021 BioMed Central Ltd.</rights><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3</citedby><cites>FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3</cites><orcidid>0000-0002-9898-5754</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788908/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2478857605?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,25734,27905,27906,36993,36994,44571,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33407818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Lei</creatorcontrib><creatorcontrib>Yao, Jingxin</creatorcontrib><creatorcontrib>Chen, Shanyu</creatorcontrib><creatorcontrib>He, Tingmei</creatorcontrib><creatorcontrib>Chai, Yijun</creatorcontrib><creatorcontrib>Zhou, Ziyao</creatorcontrib><creatorcontrib>Shi, Xiaogang</creatorcontrib><creatorcontrib>Liu, Haifeng</creatorcontrib><creatorcontrib>Zhong, Zhijun</creatorcontrib><creatorcontrib>Fu, Hualin</creatorcontrib><creatorcontrib>Peng, Guangneng</creatorcontrib><title>First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China.
A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis.
Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P < 0.05). There are also significant differences in the prevalence of Blastocystis sp. among different species (P < 0.05), and the highest of Blastocystis sp. prevalence was observed in white-cheeked gibbon, black great squirrel, and red giant flying squirrel (100%). Subtype analysis of Blastocystis sp. revealed nine subtypes, including six zoonotic STs (ST1-5, and ST8) and three animal-specific STs (ST10, ST14, and ST17), with ST17 as the predominant subtype (26/66) in Blastocystis sp.-positive isolates.
To our knowledge, this is the first report on the prevalence and subtype distribution of Blastocystis sp. among captive wildlife in zoos in southwestern China. This study highlights that these animals may serve as reservoirs for human Blastocystis sp. infections.</description><subject>Animals</subject><subject>Animals, Wild - parasitology</subject><subject>Animals, Zoo - parasitology</subject><subject>Blastocystis</subject><subject>Blastocystis - genetics</subject><subject>Blastocystis - isolation & purification</subject><subject>Blastocystis Infections - epidemiology</subject><subject>Blastocystis sp</subject><subject>captive animals</subject><subject>Captive wildlife</subject><subject>China</subject><subject>China - epidemiology</subject><subject>Deoxyribonucleic acid</subject><subject>digestive tract</subject><subject>Disease Reservoirs</subject><subject>Distribution</subject><subject>DNA</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Feces</subject><subject>Feces - parasitology</subject><subject>Flight</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>genes</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Health aspects</subject><subject>Host-parasite relationships</subject><subject>human diseases</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Infections</subject><subject>Irritable bowel syndrome</subject><subject>Microbiota</subject><subject>Nucleotide sequence</subject><subject>Parasites</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>PCR</subject><subject>Phylogenetic analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Prevalence</subject><subject>Protozoa</subject><subject>rRNA</subject><subject>Species</subject><subject>Squirrels</subject><subject>Subtypes</subject><subject>Wildlife</subject><subject>Zoo animals</subject><subject>Zoonoses</subject><subject>Zoos</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqFkluLEzEcxQdR3HX1C_ggA77ow9TcLy_CWlwtLAhenkOaS5tlmtQko9ZPb7pd160IkodJ_vmdQ-Zwuu4pBDMIBXtVIAYcDgCBARAK6YDudaeQUzZgDOj9O_uT7lEpVwAwICl72J1gTAAXUJx27iLkUvtgXazBB6NrSLHX0fabNDozjTr3ZVrW3TbEVZ98_2bUpSazKzWUvmxnfYj9z5SaJGz0WPbHkqa6_u5KdTn283WI-nH3wLdL9-Tme9Z9uXj7ef5-uPzwbjE_vxwMw6AOyHuCGfFEO24tQJpj74ggzhErKJZYCMCpZhB40SbISQHY0jpgrUeWO3zWLQ6-Nukrtc3tSXmnkg7qepDySulcgxmd8lRbKJsJMJhIQZaWWiQ01W4JnCW4eb0-eG2n5cZZ0wLKejwyPb6JYa1W6ZviXAgJRDN4cWOQ09epxaE2oRg3jjq6NBWFKCKISUrJ_1HCGURSSt7Q53-hV2nKsaW6p4SgnAH6h1rp9q8h-tSeaPam6pxRQCWQHDVq9g-qLes2waTofGjzI8HLI0FjqvtRV3oqRS0-fTxm0YE1OZWSnb-NDgK176869Fe1_qrr_qq96Nnd0G8lvwuLfwGvKeoy</recordid><startdate>20210106</startdate><enddate>20210106</enddate><creator>Deng, Lei</creator><creator>Yao, Jingxin</creator><creator>Chen, Shanyu</creator><creator>He, Tingmei</creator><creator>Chai, Yijun</creator><creator>Zhou, Ziyao</creator><creator>Shi, Xiaogang</creator><creator>Liu, Haifeng</creator><creator>Zhong, Zhijun</creator><creator>Fu, Hualin</creator><creator>Peng, Guangneng</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9898-5754</orcidid></search><sort><creationdate>20210106</creationdate><title>First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China</title><author>Deng, Lei ; Yao, Jingxin ; Chen, Shanyu ; He, Tingmei ; Chai, Yijun ; Zhou, Ziyao ; Shi, Xiaogang ; Liu, Haifeng ; Zhong, Zhijun ; Fu, Hualin ; Peng, Guangneng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Animals, Wild - parasitology</topic><topic>Animals, Zoo - parasitology</topic><topic>Blastocystis</topic><topic>Blastocystis - genetics</topic><topic>Blastocystis - isolation & purification</topic><topic>Blastocystis Infections - epidemiology</topic><topic>Blastocystis sp</topic><topic>captive animals</topic><topic>Captive wildlife</topic><topic>China</topic><topic>China - epidemiology</topic><topic>Deoxyribonucleic acid</topic><topic>digestive tract</topic><topic>Disease Reservoirs</topic><topic>Distribution</topic><topic>DNA</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Feces</topic><topic>Feces - parasitology</topic><topic>Flight</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>genes</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Health aspects</topic><topic>Host-parasite relationships</topic><topic>human diseases</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Infections</topic><topic>Irritable bowel syndrome</topic><topic>Microbiota</topic><topic>Nucleotide sequence</topic><topic>Parasites</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>PCR</topic><topic>Phylogenetic analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Prevalence</topic><topic>Protozoa</topic><topic>rRNA</topic><topic>Species</topic><topic>Squirrels</topic><topic>Subtypes</topic><topic>Wildlife</topic><topic>Zoo animals</topic><topic>Zoonoses</topic><topic>Zoos</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Lei</creatorcontrib><creatorcontrib>Yao, Jingxin</creatorcontrib><creatorcontrib>Chen, Shanyu</creatorcontrib><creatorcontrib>He, Tingmei</creatorcontrib><creatorcontrib>Chai, Yijun</creatorcontrib><creatorcontrib>Zhou, Ziyao</creatorcontrib><creatorcontrib>Shi, Xiaogang</creatorcontrib><creatorcontrib>Liu, Haifeng</creatorcontrib><creatorcontrib>Zhong, Zhijun</creatorcontrib><creatorcontrib>Fu, Hualin</creatorcontrib><creatorcontrib>Peng, Guangneng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Science in Context</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Parasites & vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Lei</au><au>Yao, Jingxin</au><au>Chen, Shanyu</au><au>He, Tingmei</au><au>Chai, Yijun</au><au>Zhou, Ziyao</au><au>Shi, Xiaogang</au><au>Liu, Haifeng</au><au>Zhong, Zhijun</au><au>Fu, Hualin</au><au>Peng, Guangneng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China</atitle><jtitle>Parasites & vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2021-01-06</date><risdate>2021</risdate><volume>14</volume><issue>1</issue><spage>11</spage><epage>11</epage><pages>11-11</pages><artnum>11</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>Blastocystis sp. is an anaerobic protozoan that parasitizes many animal hosts and the human gastrointestinal tract, and its pathogenicity is controversial. Captive wildlife may be potential reservoirs for human infection with Blastocystis sp. The present study was performed to investigate the prevalence and subtype distribution of Blastocystis sp. in zoo animals in Sichuan Province, southwestern China.
A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of Blastocystis sp. were determined by PCR amplification of the barcode region of the SSU rRNA gene and phylogenetic analysis.
Overall, 15.7% (66/420) of the animal samples and 20.7% (14/54) of the species tested were shown to be infected with Blastocystis sp. The highest prevalence of Blastocystis sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (P < 0.05). There are also significant differences in the prevalence of Blastocystis sp. among different species (P < 0.05), and the highest of Blastocystis sp. prevalence was observed in white-cheeked gibbon, black great squirrel, and red giant flying squirrel (100%). Subtype analysis of Blastocystis sp. revealed nine subtypes, including six zoonotic STs (ST1-5, and ST8) and three animal-specific STs (ST10, ST14, and ST17), with ST17 as the predominant subtype (26/66) in Blastocystis sp.-positive isolates.
To our knowledge, this is the first report on the prevalence and subtype distribution of Blastocystis sp. among captive wildlife in zoos in southwestern China. This study highlights that these animals may serve as reservoirs for human Blastocystis sp. infections.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>33407818</pmid><doi>10.1186/s13071-020-04515-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9898-5754</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1756-3305 |
| ispartof | Parasites & vectors, 2021-01, Vol.14 (1), p.11-11, Article 11 |
| issn | 1756-3305 1756-3305 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_f5ad19d820c34984bd5d28a5aeb0ed43 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Animals Animals, Wild - parasitology Animals, Zoo - parasitology Blastocystis Blastocystis - genetics Blastocystis - isolation & purification Blastocystis Infections - epidemiology Blastocystis sp captive animals Captive wildlife China China - epidemiology Deoxyribonucleic acid digestive tract Disease Reservoirs Distribution DNA DNA, Protozoan - genetics DNA, Ribosomal - genetics Feces Feces - parasitology Flight Gastrointestinal system Gastrointestinal tract genes Genetic aspects Genetic Variation Genotype Health aspects Host-parasite relationships human diseases Humans Identification and classification Infections Irritable bowel syndrome Microbiota Nucleotide sequence Parasites Pathogenicity Pathogens PCR Phylogenetic analysis Phylogenetics Phylogeny Prevalence Protozoa rRNA Species Squirrels Subtypes Wildlife Zoo animals Zoonoses Zoos |
| title | First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T17%3A12%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=First%20identification%20and%20molecular%20subtyping%20of%20Blastocystis%20sp.%20in%20zoo%20animals%20in%20southwestern%20China&rft.jtitle=Parasites%20&%20vectors&rft.au=Deng,%20Lei&rft.date=2021-01-06&rft.volume=14&rft.issue=1&rft.spage=11&rft.epage=11&rft.pages=11-11&rft.artnum=11&rft.issn=1756-3305&rft.eissn=1756-3305&rft_id=info:doi/10.1186/s13071-020-04515-2&rft_dat=%3Cgale_doaj_%3EA650590972%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c630t-2ff4364f4ae7dd02a73fe484ee4d8539388075a610f84d82e9806bde0ddf2d7e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2478857605&rft_id=info:pmid/33407818&rft_galeid=A650590972&rfr_iscdi=true |