A Richer Community of Botryosphaeriaceae Within a Less Diverse Community of Fungal Endophytes in Grapevines than in Adjacent Forest Trees Revealed by a Mixed Metabarcoding Strategy

Botryosphaeriaceae are a diverse group of endophytic fungi colonizing the inner tissue of many woody species. As opportunist pathogens, they have been increasingly involved in diebacks worldwide. Nonetheless, the diversity of Botryosphaeriaceae, especially in asymptomatic plants, remains largely unk...

Full description

Saved in:
Bibliographic Details
Published in:Phytobiomes journal 2020-01, Vol.4 (3), p.252-267
Main Authors: Laurent, Benoit, Marchand, Marylise, Chancerel, Emilie, Saint-Jean, Gilles, Capdevielle, Xavier, Poeydebat, Charlotte, Bellée, Anthony, Comont, Gwenaëlle, Villate, Laure, Desprez-Loustau, Marie-Laure
Format: Article
Language:English
Subjects:
Environmental Sciences
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Botryosphaeriaceae are a diverse group of endophytic fungi colonizing the inner tissue of many woody species. As opportunist pathogens, they have been increasingly involved in diebacks worldwide. Nonetheless, the diversity of Botryosphaeriaceae, especially in asymptomatic plants, remains largely unknown. Using an innovative and mixed strategy of metabarcoding, this study aims to investigate the diversity of the fungal endophyte community, with a focus on Botryosphaeriaceae, which colonize grapevine and adjacent oak and pine trees in a French landscape. These data were used to test if the differentiation between hosts is more important than geographical effects for shaping the Botryosphaeriaceae communities and whether that similarity is higher between communities of grapevine and oak (both Angiosperms) than between oak and pine trees. We revealed a high level of diversity in Botryosphaeriaceae fungi, in both grapevines and forest trees, with a greater richness for grapevines. Contrasting results were obtained for the endophytic community, which was more diverse in forest trees. Our results support the hypothesis that host factors prevail on geographic effects to explain the diversity of Botryosphaeriaceae at the studied spatial scale. However, the features of the agroecosystem, such as management practices, were suggested to be more important than phylogeny to structure the fungal community. This highlights the importance of management practices for the microbiome of plants. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
ISSN:2471-2906
2471-2906
DOI:10.1094/PBIOMES-01-20-0004-R