Participation of Oxidative Stress in the Activity of Compounds Isolated from Eleutherine plicata Herb

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-07, Vol.28 (14), p.5557
• Main Authors: Gomes, Antônio Rafael Quadros, Cruz, Jorddy Neves, Castro, Ana Laura Gadelha, Cordovil Brigido, Heliton Patrick, Varela, Everton Luiz Pompeu, Vale, Valdicley Vieira, Carneiro, Liliane Almeida, Ferreira, Gleison Gonçalves, Percario, Sandro, Dolabela, Maria Fâni
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Apoptosis; Catalase - metabolism; Cell cycle; Cytotoxicity; Drug dosages; eleutherine; Eleutherine plicata; eleutherol; Ethanol; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Herbal medicine; Hypotheses; Infections; isoeleutherine; Kinases; Malaria; Mice; Molecular Docking Simulation; Naphthoquinones - chemistry; Oxidation; Oxidative Stress; Plant Extracts - chemistry; Plant Extracts - pharmacology; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Toxicity; Womens health
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28145557

From , naphthoquinones, isoeleutherine, and eleutherol were isolated, and previous studies have reported the antioxidant activity of these metabolites. The present work evaluated the role of oxidative changes in mice infected with Plasmodium berghei and treated with extract, fraction, and isolated compounds, as well as to verify possible oxidative changes induced by these treatments. extracts were prepared from powder from the bulbs, which were submitted to maceration with ethanol, yielding the extract (EEEp), which was fractionated under reflux, and the dichloromethane fraction (FDMEp) was submitted for further fractionation, leading to the isolation of isoeleutherine, eleutherine, and eleutherol. The antimalarial activity was examined using the suppressive test, evaluating the following parameters of oxidative stress: trolox equivalent antioxidant capacity (TEAC), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH). Furthermore, the molecular docking of naphthoquinones, eleutherol, eleutherine, and isoeleutherine interactions with antioxidant defense enzymes was investigated, which was favorable for the formation of the receptor-ligand complex, according to the re-rank score values. Eleutherine and isoeleutherine are the ones with the lowest binding energy for catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx1), showing themselves as possible targets of these molecules in the involvement of redox balance. Data from the present study showed that treatments with stimulated an increase in antioxidant capacity and a reduction in oxidative stress in mice infected with , with naphthoquinones being responsible for reducing oxidative changes and disease severity.