Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L.) fruit

We report a feasibility study for expressing the LTB protein (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.). We produced five regenerated plants obtained on the selection medium supplemented with an antibiotic. Stabl...

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Bibliographic Details
Published in:Czech Journal of Genetics and Plant Breeding 2014-01, Vol.50 (1), p.26-31
Main Authors: LOC, Nguyen Hoang, LONG, Dang Thanh, KIM, Tae-Geum, YANG, Moon-Sik
Format: Article
Language:English
Subjects:
Antibiotics
b subunit of e. coli heat-labile enterotoxin (ltb)
E coli
Enterotoxin B
Enzyme-linked immunosorbent assay
Escherichia coli
Feasibility studies
Fruits
Ganglioside GM1
Genetic transformation
Genomes
Heat-labile enterotoxin
Hybridization
ltb gene
plant-based vaccine
Proteins
Solanum lycopersicum
tomato fruit
Tomatoes
transgenesis
Transgenic plants
Vaccines
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Summary:We report a feasibility study for expressing the LTB protein (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.). We produced five regenerated plants obtained on the selection medium supplemented with an antibiotic. Stable integrations of the LTB gene into the genome of these plants were confirmed by Southern blot hybridization. Western blot analysis showed that only two of the five T0 transgenic tomato plants expressed the pentameric LTB protein in the fruits. An enzyme-linked immunosorbent assay indicated that these two plants synthesized the LTB protein bound specifically to GM1 ganglioside, suggesting that the LTB subunits formed active pentamers. The LTB protein produced in tomatoes can be a potential candidate for inexpensive, safe, and effective plant-based vaccines.
ISSN:1212-1975
1805-9325
DOI:10.17221/77/2013-CJGPB