Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position

Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results sugge...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2017-08, Vol.22 (9), p.1427
Main Authors: Liu, Wei, Shin, Dongwon, Ng, Martin, Sanbonmatsu, Karissa Y, Tor, Yitzhak, Cooperman, Barry S
Format: Article
Language:English
Subjects:
Adenosine
atomic mutagenesis
Base Pairing
Base pairs
BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
Chemistry
Emissivity
fluorescent mRNA
Genetic Code
kinetic mechanism
Kinetics
Models, Molecular
mRNA
mRNA:tRNA base pairing
Mutagenesis
Nucleotides - genetics
Polypeptides
Protein biosynthesis
Protein synthesis
Ribosomes - genetics
RNA, Messenger - genetics
RNA, Transfer - genetics
steric constraint
tRNA
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Summary:Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5'- and 3'-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules22091427