Effect of a genetic variant in the JAZF1 gene among obesity population

• Published in: Journal of King Saud University. Science 2022-07, Vol.34 (5), p.102112, Article 102112
• Main Author: Elsadek, Mohamed Farouk
• Format: Article
• Language: English
• Subjects: Genotype assay; JAZF1; Obesity; Rs864745
• ISSN: 1018-3647
• DOI: 10.1016/j.jksus.2022.102112

Obesity is a complex condition characterized by an excess of adipose tissue and a low-grade chronic inflammation. Juxtaposed with another zinc finger protein 1 (JAZF1) is one of the genes identified through genome wide association studies which is associated in type 2 diabetes, obesity and other forms of human diseases. JAZF1 controls the expression of several genes involved in lipid and carbohydrate metabolism. Limited studies have been conducted in the obesity population and none of the studies was documented in the Saudi population. The aim of this study was to investigate the genetic association with rs864745 SNP genotype assay in JAZF1 gene in the obesity population in Saudi Arabia. This study includes 80 obese patients and 50 healthy controls. Polymerase chain reaction was performed in the rs864745 SNP of JAZF1 gene using isolated genomic DNA from 130 participants. Anthropometric measurements were calculated between cases and controls using t-tests. Hardy-Weinberg Equilibrium (HWE) analysis, genotype and allele frequencies were calculated between obesity cases and controls. Anova analysis was carried out between rs864745 SNP genotype assay and anthropmetric measurements. Statistical analysis was performed using with SPSS software. Weight, BMI, HDLC, LDLC, and TG were all associated with obesity in the clinical data (p  0.05). ANOVA analysis was not associated (p