Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion

Background Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti‐inflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti‐inflammatory effects of blood‐serum‐derived exosomes in tre...

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Bibliographic Details
Published in:Animal models and experimental medicine 2022-12, Vol.5 (4), p.362-368
Main Authors: Keivan, Mona, Mansouri Torghabeh, Fatemeh, Davoodi, Samira, Moradi Maryamneghari, Shima, Dadfar, Reza
Format: Article
Language:English
Subjects:
Anesthesia
Animals
Anti-inflammatory agents
anti‐inflammation
Apoptosis
Blood
Body fluids
Caspase
exosome
Exosomes
Gene expression
Immunohistochemistry
Infertility
Inflammation
Injection
Morphology
Motility
Orchitis
Original
Oxidative stress
parameter
Physiology
Regular
Sperm
Spermatogenesis
Testes
testicular torsion
Testosterone
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Summary:Background Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti‐inflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti‐inflammatory effects of blood‐serum‐derived exosomes in treatment of acute inflammation following TT in mice were evaluated. Materials and Methods Eighteen male mice were grouped as healthy control, TT, and TT + exosome. TT was induced surgically, and exosomes were extracted from blood serum and administrated by a single intratesticular injection (10 IU). Malondialdehyde (MDA) and Griess assays were used to evaluate the level of oxidative stress. Sperm indices, testosterone (Tes), and apoptotic gene expression (p‐53, Bcl2, and Caspase‐3) were also assessed. H&E and immunohistochemistry (IHC) stainings were used for histopathological investigations. Data analysis was applied by SPSS (v.19) software. Results Oxidative stress and apoptotic genes expression were increased significantly (p 
ISSN:2576-2095
2096-5451
2576-2095
DOI:10.1002/ame2.12232